Motor Generated Torque Drives Coupled Yawing and Orbital Rotations of Kinesin Coated Gold Nanorods

Overview

Journal Commun Biol

Specialty Biology

Date 2022 Dec 20

PMID 36539506

Authors

Mitsuhiro Sugawa

Yohei Maruyama

Masahiko Yamagishi

Robert A Cross

Junichiro Yajima

Affiliations

Soon will be listed here.

Abstract

Kinesin motor domains generate impulses of force and movement that have both translational and rotational (torque) components. Here, we ask how the torque component influences function in cargo-attached teams of weakly processive kinesins. Using an assay in which kinesin-coated gold nanorods (kinesin-GNRs) translocate on suspended microtubules, we show that for both single-headed KIF1A and dimeric ZEN-4, the intensities of polarized light scattered by the kinesin-GNRs in two orthogonal directions periodically oscillate as the GNRs crawl towards microtubule plus ends, indicating that translocating kinesin-GNRs unidirectionally rotate about their short (yaw) axes whilst following an overall left-handed helical orbit around the microtubule axis. For orientations of the GNR that generate a signal, the period of this short axis rotation corresponds to two periods of the overall helical trajectory. Torque force thus drives both rolling and yawing of near-spherical cargoes carrying rigidly-attached weakly processive kinesins, with possible relevance to intracellular transport.

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