Four-dimensional Spatial Nanometry of Single Particles in Living Cells Using Polarized Quantum Rods

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2013 Aug 13

PMID 23931303

Citations 10

Authors

Tomonobu M Watanabe

Fumihiko Fujii

Takashi Jin

Eiji Umemoto

Masayuki Miyasaka

Hideaki Fujita

Toshio Yanagida

Affiliations

Soon will be listed here.

Abstract

Single particle tracking is widely used to study protein movement with high spatiotemporal resolution both in vitro and in cells. Quantum dots, which are semiconductor nanoparticles, have recently been employed in single particle tracking because of their intense and stable fluorescence. Although single particles inside cells have been tracked in three spatial dimensions (X, Y, Z), measurement of the angular orientation of a molecule being tracked would significantly enhance our understanding of the molecule's function. In this study, we synthesized highly polarized, rod-shaped quantum dots (Qrods) and developed a coating method that optimizes the Qrods for biological imaging. We describe a Qrod-based single particle tracking technique that blends optical nanometry with nanomaterial science to simultaneously measure the three-dimensional and angular movements of molecules. Using Qrods, we spatially tracked a membrane receptor in living cells in four dimensions with precision close to the single-digit range in nanometers and degrees.

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