Assessment of Tricalcium Phosphate/titanium Dioxide (TCP/TiO2) Nanocomposite Scaffold Compared with Bone Autograft and Hydroxyapatite (HA) on the Healing of Segmental Femur Bone Defect in Rabbits

Overview

Journal J Mater Sci Mater Med

Publisher Springer

Specialty Biomedical Engineering

Date 2022 Dec 8

PMID 36480067

Authors

Hoseyn Sonbolekar

Jahandideh Alireza

Asghary Ahmad

Saeed Hesaraki

Abolfazl Akbarzadeh

Affiliations

Soon will be listed here.

Abstract

Bone healing is a tissue process after a surgical operation. Many formulated materials have been designed for improving these procedures. The purpose of this study was to evaluate the effectiveness of nanocomposite tricalcium phosphate scaffolds combined with Titanium dioxide scaffold (TCP/TiO2) for femoral defects regeneration in rabbits. We studied 80 mature male New Zealand white rabbits weighing between 3 and 3.5 kg. Rabbits were subdivided into four groups. Anesthesia was performed before surgical operation by 50 mg/kg Ketamine 10% and 5 mg/kg xylazine 5% intramuscularly. We inducted a 6 × 5 mm diameter cylinder defect on the femur. Animals were separated into four trial groups of 20 animals each. After defecting, the experimental groups include control, autograft, hydroxyapatite, and TCP/TiO2 (received pure nanocomposite TCP/TiO2 material). A pathologist evaluated the sections on days 15, 30, 45, and 60 after surgery. The improvement of new and lamellar bone formation was the best in the nanocomposite TCP/TiO2 group at various point times, especially 60 days after surgery. We found that TCP/TiO2 nanocomposite has a significant improving function in the remodeling of bone in the defect areas. Graphical abstract.

Citing Articles

Porous Mg-Zn-Ca scaffolds for bone repair: a study on microstructure, mechanical properties and in vitro degradation behavior.

Huo L, Li Q, Jiang L, Jiang H, Zhao J, Yang K J Mater Sci Mater Med. 2024; 35(1):22.

PMID: 38526601 PMC: 10963566. DOI: 10.1007/s10856-023-06754-y.

References

Weiss P, Gauthier O, Bouler J, Grimandi G, Daculsi G . Injectable bone substitute using a hydrophilic polymer. Bone. 1999; 25(2 Suppl):67S-70S. DOI: 10.1016/s8756-3282(99)00146-5. View

Whitaker R, Hernaez-Estrada B, Hernandez R, Santos-Vizcaino E, Spiller K . Immunomodulatory Biomaterials for Tissue Repair. Chem Rev. 2021; 121(18):11305-11335. DOI: 10.1021/acs.chemrev.0c00895. View

Toriello M, Afsari M, Shon H, Tijing L . Progress on the Fabrication and Application of Electrospun Nanofiber Composites. Membranes (Basel). 2020; 10(9). PMC: 7559347. DOI: 10.3390/membranes10090204. View

Dimitriou R, Mataliotakis G, Angoules A, Kanakaris N, Giannoudis P . Complications following autologous bone graft harvesting from the iliac crest and using the RIA: a systematic review. Injury. 2011; 42 Suppl 2:S3-15. DOI: 10.1016/j.injury.2011.06.015. View

Shao A, Ling Y, Xu L, Liu S, Fan C, Wang Z . Xenogeneic bone matrix immune risk assessment using GGTA1 knockout mice. Artif Cells Nanomed Biotechnol. 2018; 46(sup3):S359-S369. DOI: 10.1080/21691401.2018.1493489. View

Sieger D, Korzinskas T, Jung O, Stojanovic S, Wenisch S, Smeets R . The Addition of High Doses of Hyaluronic Acid to a Biphasic Bone Substitute Decreases the Proinflammatory Tissue Response. Int J Mol Sci. 2019; 20(8). PMC: 6515558. DOI: 10.3390/ijms20081969. View

Ghanaati S, Barbeck M, Hilbig U, Hoffmann C, Unger R, Sader R . An injectable bone substitute composed of beta-tricalcium phosphate granules, methylcellulose and hyaluronic acid inhibits connective tissue influx into its implantation bed in vivo. Acta Biomater. 2011; 7(11):4018-28. DOI: 10.1016/j.actbio.2011.07.003. View

Barbeck M, Hoffmann C, Sader R, Peters F, Hubner W, Kirkpatrick C . Injectable Bone Substitute Based on β-TCP Combined With a Hyaluronan-Containing Hydrogel Contributes to Regeneration of a Critical Bone Size Defect Towards Restitutio ad Integrum. J Oral Implantol. 2015; 42(2):127-37. DOI: 10.1563/aaid-joi-D-14-00203. View

Mohseni M, Jahandideh A, Abedi G, Akbarzadeh A, Hesaraki S . Assessment of tricalcium phosphate/collagen (TCP/collagene)nanocomposite scaffold compared with hydroxyapatite (HA) on healing of segmental femur bone defect in rabbits. Artif Cells Nanomed Biotechnol. 2017; 46(2):242-249. DOI: 10.1080/21691401.2017.1324463. View

10.

Hidalgo-Bastida L, Cartmell S . Mesenchymal stem cells, osteoblasts and extracellular matrix proteins: enhancing cell adhesion and differentiation for bone tissue engineering. Tissue Eng Part B Rev. 2010; 16(4):405-12. DOI: 10.1089/ten.TEB.2009.0714. View

11.

Arner J, Santrock R . A historical review of common bone graft materials in foot and ankle surgery. Foot Ankle Spec. 2014; 7(2):143-51. DOI: 10.1177/1938640013516358. View

12.

Clough B, McCarley M, Krause U, Zeitouni S, Froese J, Mcneill E . Bone regeneration with osteogenically enhanced mesenchymal stem cells and their extracellular matrix proteins. J Bone Miner Res. 2014; 30(1):83-94. PMC: 4280327. DOI: 10.1002/jbmr.2320. View

13.

Schaaf H, Lendeckel S, Howaldt H, Streckbein P . Donor site morbidity after bone harvesting from the anterior iliac crest. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010; 109(1):52-8. DOI: 10.1016/j.tripleo.2009.08.023. View

14.

Kim H, Cha J, Jang M, Kim P . Hyaluronic acid-based extracellular matrix triggers spontaneous M2-like polarity of monocyte/macrophage. Biomater Sci. 2019; 7(6):2264-2271. DOI: 10.1039/c9bm00155g. View

15.

Liu M, Zeng X, Ma C, Yi H, Ali Z, Mou X . Injectable hydrogels for cartilage and bone tissue engineering. Bone Res. 2017; 5:17014. PMC: 5448314. DOI: 10.1038/boneres.2017.14. View

16.

Barbeck M, Serra T, Booms P, Stojanovic S, Najman S, Engel E . Analysis of the degradation and the tissue response to bi-layered 3D-printed scaffolds combining PLA and biphasic PLA/bioglass components - Guidance of the inflammatory response as basis for osteochondral regeneration. Bioact Mater. 2018; 2(4):208-223. PMC: 5935508. DOI: 10.1016/j.bioactmat.2017.06.001. View

17.

Shoaib M, Saeed A, Akhtar J, Rahman M, Ullah A, Jurkschat K . Potassium-doped mesoporous bioactive glass: Synthesis, characterization and evaluation of biomedical properties. Mater Sci Eng C Mater Biol Appl. 2017; 75:836-844. DOI: 10.1016/j.msec.2017.02.090. View

18.

Yamada M, Egusa H . Current bone substitutes for implant dentistry. J Prosthodont Res. 2017; 62(2):152-161. DOI: 10.1016/j.jpor.2017.08.010. View

19.

Low K, Tan S, Sharif Zein S, Roether J, Mourino V, Boccaccini A . Calcium phosphate-based composites as injectable bone substitute materials. J Biomed Mater Res B Appl Biomater. 2010; 94(1):273-86. DOI: 10.1002/jbm.b.31619. View

20.

ALLEN H, WASE A, Bear W . Indomethacin and aspirin: effect of nonsteroidal anti-inflammatory agents on the rate of fracture repair in the rat. Acta Orthop Scand. 1980; 51(4):595-600. DOI: 10.3109/17453678008990848. View