Molecular Detection of in Cats in China

Overview

Journal Animals (Basel)

Date 2022 Nov 26

PMID 36428294

Authors

Fangyuan Yin

Daoe Mu

Zhuojia Tian

Dong Li

Xiting Ma

Jinming Wang

Guiquan Guan

Hong Yin

Facai Li

Affiliations

Soon will be listed here.

Abstract

As there are few studies of spp. infection in cats in China, or anywhere in the world, the aim of this study was to explore the epidemic features of babesiosis in pet cats in China. In total, 429 blood samples were randomly collected in four different geographical regions. The 18S rRNA gene fragment of spp. was amplified by nest polymerase chain reaction (PCR), and haplotype and phylogenetic analysis of were performed to analyze the relationship of this protozoa. The total positive rate of infection was 2.8%. BLAST analysis indicated that was detected in 12 cats. Among these, 4.3%, 3.1%, 0.8% and 2.0% were from Chongqing, Fujian, Hubei and Shandong, respectively. Haplotype and phylogenetic analysis showed that there were nine haplotypes and no obvious genetic variation among populations. These findings will be helpful for understanding the epidemiology of spp. in China, and provide a foundation for developing effective preventative strategies.

Citing Articles

Feline vector-borne haemopathogens in Türkiye: the first molecular detection of Mycoplasma wenyonii and ongoing Babesia ovis DNA presence in unspecific hosts.

Ceylan O, Ma Z, Ceylan C, Ider M, Evci A, Mavinehir A BMC Vet Res. 2024; 20(1):365.

PMID: 39143614 PMC: 11323478. DOI: 10.1186/s12917-024-04209-2.

Molecular epidemiological investigation of piroplasms carried by pet cats and dogs in an animal hospital in Guiyang, China.

Wu S, Meng J, Yu F, Zhou C, Yang B, Chen X Front Microbiol. 2023; 14:1266583.

PMID: 37901826 PMC: 10602747. DOI: 10.3389/fmicb.2023.1266583.

Infection in a Cat with Immune-Mediated Haemolytic Anaemia and Thrombocytopenia.

Almendros A, Choi Y, Beczkowski P, Baiker K, Barrs V, Beatty J Animals (Basel). 2023; 13(13).

PMID: 37443927 PMC: 10339935. DOI: 10.3390/ani13132128.

Correction: Yin et al. Molecular Detection of in Cats in China. 2022, , 3066.

Yin F, Mu D, Tian Z, Li D, Ma X, Wang J Animals (Basel). 2023; 13(1).

PMID: 36611794 PMC: 9817781. DOI: 10.3390/ani13010138.

References

Xu D, Zhang J, Shi Z, Song C, Zheng X, Zhang Y . Molecular detection of vector-borne agents in dogs from ten provinces of China. Parasit Vectors. 2015; 8:501. PMC: 4589947. DOI: 10.1186/s13071-015-1120-y. View

Simking P, Wongnakphet S, Stich R, Jittapalapong S . Detection of Babesia vogeli in stray cats of metropolitan Bangkok, Thailand. Vet Parasitol. 2010; 173(1-2):70-5. DOI: 10.1016/j.vetpar.2010.06.025. View

Cao J, Yang Q, Zhang J, Zhou Y, Zhang H, Gong H . Seroprevalence survey of Babesia gibsoni infection and tick species in dogs in East China. Vet Parasitol. 2015; 214(1-2):12-5. DOI: 10.1016/j.vetpar.2015.10.002. View

Stegeman J, Birkenheuer A, Kruger J, Breitschwerdt E . Transfusion-associated Babesia gibsoni infection in a dog. J Am Vet Med Assoc. 2003; 222(7):959-63, 952. DOI: 10.2460/javma.2003.222.959. View

Ayoob A, Prittie J, Hackner S . Feline babesiosis. J Vet Emerg Crit Care (San Antonio). 2010; 20(1):90-7. DOI: 10.1111/j.1476-4431.2009.00493.x. View

Koster L, Lobetti R, Kelly P . Canine babesiosis: a perspective on clinical complications, biomarkers, and treatment. Vet Med (Auckl). 2018; 6:119-128. PMC: 6065580. DOI: 10.2147/VMRR.S60431. View

Wang J, Wang X, Sun H, Lv Z, Li Y, Luo J . Molecular evidence of piroplasm infection in companion animals in Hunan Province, China. BMC Vet Res. 2020; 16(1):297. PMC: 7433179. DOI: 10.1186/s12917-020-02500-6. View

Mathe A, Voros K, Papp L, Reiczigel J . Clinical manifestations of canine babesiosis in Hungary (63 cases). Acta Vet Hung. 2006; 54(3):367-85. DOI: 10.1556/AVet.54.2006.3.7. View

Vilhena H, Tvarijonaviciute A, Ceron J, Vieira L, Pastor J, Silvestre-Ferreira A . Acute phase proteins response in cats naturally infected with Hepatozoon felis and Babesia vogeli. Vet Clin Pathol. 2017; 46(1):72-76. DOI: 10.1111/vcp.12451. View

10.

Hartmann K, Addie D, Belak S, Boucraut-Baralon C, Egberink H, Frymus T . Babesiosis in cats: ABCD guidelines on prevention and management. J Feline Med Surg. 2013; 15(7):643-6. PMC: 11148951. DOI: 10.1177/1098612X13489230. View

11.

Bawm S, Myaing T, Thu M, Akter S, Htun L, Win M . PCR detection and genetic characterization of piroplasms from dogs in Myanmar, and a possible role of dogs as reservoirs for Theileria parasites infecting cattle, water buffaloes, and goats. Ticks Tick Borne Dis. 2021; 12(4):101729. DOI: 10.1016/j.ttbdis.2021.101729. View

12.

Kelly P, Koster L, Li J, Zhang J, Huang K, Branford G . Survey of vector-borne agents in feral cats and first report of Babesia gibsoni in cats on St Kitts, West Indies. BMC Vet Res. 2017; 13(1):331. PMC: 5683591. DOI: 10.1186/s12917-017-1230-1. View

13.

Yang J, Li Y, Liu Z, Liu J, Guan G, Chen Z . Molecular evidence for piroplasms in wild Reeves' muntjac (Muntiacus reevesi) in China. Parasitol Int. 2014; 63(5):713-6. DOI: 10.1016/j.parint.2014.06.002. View

14.

Solano-Gallego L, Sainz A, Roura X, Estrada-Pena A, Miro G . A review of canine babesiosis: the European perspective. Parasit Vectors. 2016; 9(1):336. PMC: 4902949. DOI: 10.1186/s13071-016-1596-0. View

15.

Wong S, Poon R, Hui J, Yuen K . Detection of Babesia hongkongensis sp. nov. in a free-roaming Felis catus cat in Hong Kong. J Clin Microbiol. 2012; 50(8):2799-803. PMC: 3421483. DOI: 10.1128/JCM.01300-12. View

16.

Passos L, Geiger S, Ribeiro M, Pfister K, Zahler-Rinder M . First molecular detection of Babesia vogeli in dogs from Brazil. Vet Parasitol. 2004; 127(1):81-5. DOI: 10.1016/j.vetpar.2004.07.028. View

17.

Zhang Y, Zhang X, Liu J . Ticks (Acari: Ixodoidea) in China: Geographical distribution, host diversity, and specificity. Arch Insect Biochem Physiol. 2019; 102(3):e21544. PMC: 6850514. DOI: 10.1002/arch.21544. View

18.

Slenning B . Global climate change and implications for disease emergence. Vet Pathol. 2010; 47(1):28-33. DOI: 10.1177/0300985809354465. View

19.

Niu Q, Yang J, Liu Z, Gao S, Pan Y, Guan G . First Molecular Detection of Piroplasm Infection in Pet Dogs from Gansu, China. Front Microbiol. 2017; 8():1029. PMC: 5461281. DOI: 10.3389/fmicb.2017.01029. View

20.

Morelli S, Diakou A, Di Cesare A, Colombo M, Traversa D . Canine and Feline Parasitology: Analogies, Differences, and Relevance for Human Health. Clin Microbiol Rev. 2021; 34(4):e0026620. PMC: 8404700. DOI: 10.1128/CMR.00266-20. View