Studying T Cell Development in Neonatal and Adult Thymic Slices

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2022 Nov 14

PMID 36374461

Authors

Laura L McIntyre

Lydia K Lutes

Ellen A Robey

Affiliations

Soon will be listed here.

Abstract

T cell development occurs in the thymus and is coordinated temporally and spatially within the highly complex thymic microenvironment. Therefore, T cell selection and maturation events cannot be fully recapitulated using traditional two-dimensional tissue culture in vitro. The thymic slice system provides a highly versatile system for studying T cell development ex vivo while preserving three-dimensional thymic architecture. Using the thymic slice system, T cell selection and maturation events can be visualized by live imaging and quantified by flow cytometry. Here we describe the process for generating slices from neonatal and adult mice.

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