CRISPR/Cas13a Combined with Hybridization Chain Reaction for Visual Detection of Influenza A (H1N1) Virus

Overview

Journal Anal Bioanal Chem

Specialty Chemistry

Date 2022 Oct 20

PMID 36264297

Authors

Hongyu Zhou

Shengjun Bu

Yao Xu

Lulu Xue

Zhongyi Li

Zhuo Hao

Jiayu Wan

Feng Tang

Affiliations

Soon will be listed here.

Abstract

This study provides proof of concept of a colorimetric biosensor for influenza H1N1 virus assay based on the CRISPR/Cas13a system and hybridization chain reaction (HCR). Target RNA of influenza H1N1 virus activated the trans-cleavage activity of Cas13a, which cleaved the special RNA sequence (-UUU-) of the probe, further initiating HCR to copiously generate G-rich DNA. Abundant G-quadruplex/hemin was formed in the presence of hemin, thus catalyzing a colorimetric reaction. The colorimetric biosensor exhibited a linear relationship from 10 pM to 100 nM. The detection limit was 0.152 pM. The biosensor specificity was excellent. This new and sensitive detection method for influenza virus is a promising rapid influenza diagnostic test.

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