Clustered Regularly Interspaced Short Palindromic Repeats-Based Microfluidic System in Infectious Diseases Diagnosis: Current Status, Challenges, and Perspectives

Overview

Journal Adv Sci (Weinh)

Specialty Biomedical Engineering

Date 2022 Oct 18

PMID 36257813

Authors

Yi Xie

Huimin Li

Fumin Chen

Srisruthi Udayakumar

Khyati Arora

Hui Chen

Yang Lan

Qinqin Hu

Xiaonong Zhou

Xiaokui Guo

Leshan Xiu

Kun Yin

Affiliations

Soon will be listed here.

Abstract

Mitigating the spread of global infectious diseases requires rapid and accurate diagnostic tools. Conventional diagnostic techniques for infectious diseases typically require sophisticated equipment and are time consuming. Emerging clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) detection systems have shown remarkable potential as next-generation diagnostic tools to achieve rapid, sensitive, specific, and field-deployable diagnoses of infectious diseases, based on state-of-the-art microfluidic platforms. Therefore, a review of recent advances in CRISPR-based microfluidic systems for infectious diseases diagnosis is urgently required. This review highlights the mechanisms of CRISPR/Cas biosensing and cutting-edge microfluidic devices including paper, digital, and integrated wearable platforms. Strategies to simplify sample pretreatment, improve diagnostic performance, and achieve integrated detection are discussed. Current challenges and future perspectives contributing to the development of more effective CRISPR-based microfluidic diagnostic systems are also proposed.

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References

Wu H, Qian C, Wu C, Wang Z, Wang D, Ye Z . End-point dual specific detection of nucleic acids using CRISPR/Cas12a based portable biosensor. Biosens Bioelectron. 2020; 157:112153. DOI: 10.1016/j.bios.2020.112153. View

Xie Y, Li H, Chen F, Udayakumar S, Arora K, Chen H . Clustered Regularly Interspaced short palindromic repeats-Based Microfluidic System in Infectious Diseases Diagnosis: Current Status, Challenges, and Perspectives. Adv Sci (Weinh). 2022; 9(34):e2204172. PMC: 9731715. DOI: 10.1002/advs.202204172. View

Chen Y, Mei Y, Zhao X, Jiang X . Reagents-Loaded, Automated Assay that Integrates Recombinase-Aided Amplification and Cas12a Nucleic Acid Detection for a Point-of-Care Test. Anal Chem. 2020; 92(21):14846-14852. DOI: 10.1021/acs.analchem.0c03883. View

Huang D, Ni D, Fang M, Shi Z, Xu Z . Microfluidic Ruler-Readout and CRISPR Cas12a-Responded Hydrogel-Integrated Paper-Based Analytical Devices (μReaCH-PAD) for Visible Quantitative Point-of-Care Testing of Invasive Fungi. Anal Chem. 2021; 93(50):16965-16973. DOI: 10.1021/acs.analchem.1c04649. View

Thatcher S . DNA/RNA preparation for molecular detection. Clin Chem. 2014; 61(1):89-99. DOI: 10.1373/clinchem.2014.221374. View

Berlanda S, Breitfeld M, Dietsche C, Dittrich P . Recent Advances in Microfluidic Technology for Bioanalysis and Diagnostics. Anal Chem. 2020; 93(1):311-331. DOI: 10.1021/acs.analchem.0c04366. View

Jain S, Self W, Wunderink R, Fakhran S, Balk R, Bramley A . Community-Acquired Pneumonia Requiring Hospitalization among U.S. Adults. N Engl J Med. 2015; 373(5):415-27. PMC: 4728150. DOI: 10.1056/NEJMoa1500245. View

Bandodkar A, Gutruf P, Choi J, Lee K, Sekine Y, Reeder J . Battery-free, skin-interfaced microfluidic/electronic systems for simultaneous electrochemical, colorimetric, and volumetric analysis of sweat. Sci Adv. 2019; 5(1):eaav3294. PMC: 6357758. DOI: 10.1126/sciadv.aav3294. View

Anderson E, Haupt A, Schiel J, Chou E, Machado H, Strezoska Z . Systematic analysis of CRISPR-Cas9 mismatch tolerance reveals low levels of off-target activity. J Biotechnol. 2015; 211:56-65. DOI: 10.1016/j.jbiotec.2015.06.427. View

10.

Xiang X, Qiu D, Hegele R, Tan W . Comparison of different methods of total RNA extraction for viral detection in sputum. J Virol Methods. 2001; 94(1-2):129-35. DOI: 10.1016/s0166-0934(01)00284-1. View

11.

Rohrman B, Richards-Kortum R . A paper and plastic device for performing recombinase polymerase amplification of HIV DNA. Lab Chip. 2012; 12(17):3082-8. PMC: 3569001. DOI: 10.1039/c2lc40423k. View

12.

Shinoda H, Taguchi Y, Nakagawa R, Makino A, Okazaki S, Nakano M . Amplification-free RNA detection with CRISPR-Cas13. Commun Biol. 2021; 4(1):476. PMC: 8055673. DOI: 10.1038/s42003-021-02001-8. View

13.

Shaman J . Pandemic preparedness and forecast. Nat Microbiol. 2018; 3(3):265-267. PMC: 7097585. DOI: 10.1038/s41564-018-0117-7. View

14.

Barnes K, Lachenauer A, Nitido A, Siddiqui S, Gross R, Beitzel B . Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time. Nat Commun. 2020; 11(1):4131. PMC: 7431545. DOI: 10.1038/s41467-020-17994-9. View

15.

Borysiak M, Kimura K, Posner J . NAIL: Nucleic Acid detection using Isotachophoresis and Loop-mediated isothermal amplification. Lab Chip. 2015; 15(7):1697-707. DOI: 10.1039/c4lc01479k. View

16.

Swarts D, van der Oost J, Jinek M . Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a. Mol Cell. 2017; 66(2):221-233.e4. PMC: 6879319. DOI: 10.1016/j.molcel.2017.03.016. View

17.

Wright A, Nunez J, Doudna J . Biology and Applications of CRISPR Systems: Harnessing Nature's Toolbox for Genome Engineering. Cell. 2016; 164(1-2):29-44. DOI: 10.1016/j.cell.2015.12.035. View

18.

Kim J, Campbell A, de Avila B, Wang J . Wearable biosensors for healthcare monitoring. Nat Biotechnol. 2019; 37(4):389-406. PMC: 8183422. DOI: 10.1038/s41587-019-0045-y. View

19.

Nielsen A, Beauchamp M, Nordin G, Woolley A . 3D Printed Microfluidics. Annu Rev Anal Chem (Palo Alto Calif). 2019; 13(1):45-65. PMC: 7282950. DOI: 10.1146/annurev-anchem-091619-102649. View

20.

Orlandi P, Lampel K . Extraction-free, filter-based template preparation for rapid and sensitive PCR detection of pathogenic parasitic protozoa. J Clin Microbiol. 2000; 38(6):2271-7. PMC: 86779. DOI: 10.1128/JCM.38.6.2271-2277.2000. View