Depth Random-access Two-photon Bessel Light-sheet Imaging in Brain Tissue

Overview

Journal Opt Express

Specialties Biophysics
Ophthalmology

Date 2022 Oct 14

PMID 36236832

Authors

Dongli Xu

Jun B Ding

Leilei Peng

Affiliations

Soon will be listed here.

Abstract

Two-photon light-sheet fluorescence microscopy enables high-resolution imaging of neural activity in brain tissue at a high frame rate. Traditionally, light-sheet microscopy builds up a 3D stack by multiple depth scans with uniform spatial intervals, which substantially limits the volumetric imaging speed. Here, we introduce the depth random-access light-sheet microscopy, allowing rapid switching scanning depth for light-sheet imaging. With a low-cost electrically tunable lens and minimum modification of an existing two-photon light-sheet imaging instrument, we demonstrated fast random depth hopping light-sheet imaging at 100 frames per second in the live brain slice. Through depth random-access, calcium activities for an astrocyte were recorded on four user-selected detection planes at a refreshing rate of 25 Hz.

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