Structured Illumination Imaging with Quasi-periodic Patterns

Overview

Journal J Biophotonics

Date 2020 Feb 27

PMID 32101369

Citations 1

Authors

Dongli Xu

Jun Ding

Leilei Peng

Affiliations

Soon will be listed here.

Abstract

Structured illumination microscopy (SIM) is a well-established method for optical sectioning and super-resolution. The core of structured illumination is using a periodic pattern to excite image signals. This work reports a method for estimating minor pattern distortions from the raw image data and correcting these distortions during SIM image processing. The method was tested with both simulated and experimental image data from two-photon Bessel light-sheet SIM. The results proves the method is effective in challenging situations, where strong scattering background exists, signal-to-noise ratio (SNR) is low and the sample structure is sparse. Experimental results demonstrate restoring synaptic structures in deep brain tissue, despite the presence of strong light scattering and tissue-induced SIM pattern distortion.

Citing Articles

Depth random-access two-photon Bessel light-sheet imaging in brain tissue.

Xu D, Ding J, Peng L Opt Express. 2022; 30(15):26396-26406.

PMID: 36236832 PMC: 9363024. DOI: 10.1364/OE.456871.

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