» Articles » PMID: 36232980

Resveratrol Mitigates Oxygen and Glucose Deprivation-Induced Inflammation, NLRP3 Inflammasome, and Oxidative Stress in 3D Neuronal Culture

Overview
Journal Int J Mol Sci
Publisher MDPI
Date 2022 Oct 14
PMID 36232980
Authors
Affiliations
Soon will be listed here.
Abstract

Oxygen glucose deprivation (OGD) can produce hypoxia-induced neurotoxicity and is a mature in vitro model of hypoxic cell damage. Activated AMP-activated protein kinase (AMPK) regulates a downstream pathway that substantially increases bioenergy production, which may be a key player in physiological energy and has also been shown to play a role in regulating neuroprotective processes. Resveratrol is an effective activator of AMPK, indicating that it may have therapeutic potential as a neuroprotective agent. However, the mechanism by which resveratrol achieves these beneficial effects in SH-SY5Y cells exposed to OGD-induced inflammation and oxidative stress in a 3D gelatin scaffold remains unclear. Therefore, in the present study, we investigated the effect of resveratrol in 3D gelatin scaffold cells to understand its neuroprotective effects on NF-κB signaling, NLRP3 inflammasome, and oxidative stress under OGD conditions. Here, we show that resveratrol improves the expression levels of cell viability, inflammatory cytokines (TNF-α, IL-1, and IL-18), NF-κB signaling, and NLRP3 inflammasome, that OGD increases. In addition, resveratrol rescued oxidative stress, nuclear factor-erythroid 2 related factor 2 (Nrf2), and Nrf2 downstream antioxidant target genes (e.g., SOD, Gpx GSH, catalase, and HO-1). Treatment with resveratrol can significantly normalize OGD-induced changes in SH-SY5Y cell inflammation, oxidative stress, and oxidative defense gene expression; however, these resveratrol protective effects are affected by AMPK antagonists (Compounds C) blocking. These findings improve our understanding of the mechanism of the AMPK-dependent protective effect of resveratrol under 3D OGD-induced inflammation and oxidative stress-mediated cerebral ischemic stroke conditions.

Citing Articles

Resveratrol-Enhanced Human Neural Stem Cell-Derived Exosomes Mitigate MPP+-Induced Neurotoxicity Through Activation of AMPK and Nrf2 Pathways and Inhibition of the NLRP3 Inflammasome in SH-SY5Y Cells.

Chiang M, Yang Y, Nicol C, Chiang T, Yen C Life (Basel). 2025; 15(2).

PMID: 40003703 PMC: 11856727. DOI: 10.3390/life15020294.


Regulatory Role and Cytoprotective Effects of Exogenous Recombinant SELENOM under Ischemia-like Conditions and Glutamate Excitotoxicity in Cortical Cells In Vitro.

Turovsky E, Plotnikov E, Varlamova E Biomedicines. 2024; 12(8).

PMID: 39200220 PMC: 11351740. DOI: 10.3390/biomedicines12081756.


3,3',4,5'-Tetramethoxy-trans-stilbene and 3,4',5-trimethoxy-trans-stilbene prevent oxygen-glucose deprivation-induced injury in brain endothelial cell.

Zhou C, Zhou Y, Vong C, Khan H, Cheang W J Cell Mol Med. 2024; 28(16):e70008.

PMID: 39153195 PMC: 11330235. DOI: 10.1111/jcmm.70008.


A comprehensive review on the neuroprotective potential of resveratrol in ischemic stroke.

Owjfard M, Rahimian Z, Karimi F, Borhani-Haghighi A, Mallahzadeh A Heliyon. 2024; 10(14):e34121.

PMID: 39082038 PMC: 11284444. DOI: 10.1016/j.heliyon.2024.e34121.


Mitochondrial Oxidative Stress Regulates FOXP3+ T-Cell Activity and CD4-Mediated Inflammation in Older Adults with Frailty.

Gill J, Bansal B, Guo K, Huang F, Singh H, Hur J Int J Mol Sci. 2024; 25(11).

PMID: 38892421 PMC: 11173216. DOI: 10.3390/ijms25116235.


References
1.
Chiang M, Nicol C, Lin C, Chen S, Yen C, Huang R . Nanogold induces anti-inflammation against oxidative stress induced in human neural stem cells exposed to amyloid-beta peptide. Neurochem Int. 2021; 145:104992. DOI: 10.1016/j.neuint.2021.104992. View

2.
Wang H, Liao S, Li H, Chen Y, Yu J . Long Non-coding RNA TUG1 Sponges Mir-145a-5p to Regulate Microglial Polarization After Oxygen-Glucose Deprivation. Front Mol Neurosci. 2019; 12:215. PMC: 6748346. DOI: 10.3389/fnmol.2019.00215. View

3.
Zhu H, Jian Z, Zhong Y, Ye Y, Zhang Y, Hu X . Janus Kinase Inhibition Ameliorates Ischemic Stroke Injury and Neuroinflammation Through Reducing NLRP3 Inflammasome Activation JAK2/STAT3 Pathway Inhibition. Front Immunol. 2021; 12:714943. PMC: 8339584. DOI: 10.3389/fimmu.2021.714943. View

4.
Rahman M, Akter R, Bhattacharya T, Abdel-Daim M, Alkahtani S, Arafah M . Resveratrol and Neuroprotection: Impact and Its Therapeutic Potential in Alzheimer's Disease. Front Pharmacol. 2021; 11:619024. PMC: 7804889. DOI: 10.3389/fphar.2020.619024. View

5.
Andrade S, Ramalho M, Pereira M, Loureiro J . Resveratrol Brain Delivery for Neurological Disorders Prevention and Treatment. Front Pharmacol. 2018; 9:1261. PMC: 6262174. DOI: 10.3389/fphar.2018.01261. View