MT1 Melatonin Receptor Reconstitution in Nanodiscs

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2022 Sep 30

PMID 36180690

Authors

Jean A Boutin

Christel Logez

Marjorie Damian

Renaud Wagner

Jean-Louis Baneres

Gilles Ferry

Affiliations

Soon will be listed here.

Abstract

A way to study G protein-coupled receptors in a minimal system is to reconstruct artificial membrane mimics, made of detergent and/or of lipids in which the purified receptor is maintained. In particular, it is now possible to generate lipid nanoparticles, such as nanodiscs, in which a single receptor molecule is included. Such objects offer the invaluable potential of studying an isolated receptor stabilized in a finely controlled membrane-like environment to evaluate its pharmacology, its function, and its structure at the molecular level. In this chapter, we detail the different steps from the extraction and isolation of a recombinant MT1 melatonin receptor in detergent, down to its reconstitution into nanodiscs. A G protein activation test is further described in order to exemplify how the functionality of such particles may be investigated.

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