Exploring the Metabolome of Seminal Plasma in Two Different Horse Types: Light Versus Draft Stallions

Overview

Journal Reprod Domest Anim

Specialties Biology
Reproductive Medicine
Veterinary Medicine

Date 2022 Sep 24

PMID 36151924

Authors

Marilena Bazzano

Chenglin Zhu

Fulvio Laus

Andrea Di Giambattista

Luca Laghi

Affiliations

Soon will be listed here.

Abstract

The application of the 'omics' studies in the field of animal reproduction has been aimed at identifying novel biomarkers of fertility since the last few years. When assessing reproductive efficiency in horses, breed should also be taken into account as it can influence semen quality and fertility. Considering the growing interest in metabolomic analysis to evaluate male fertility, we aimed to investigate the metabolomic profile of seminal plasma in two different horse breeds. Twelve healthy stallions, n.6 American Quarter Horse (AQH) and n.6 Italian Draft Horse (IDH) stallions, regularly used for artificial insemination, were included in the study. Two semen collections, performed 30-day apart, were considered for the assessment of semen parameters including gel-free volume, spermatozoa (spz) concentration, spz progressive motility and seminal plasma analysis by H-NMR.Semen characteristics differed between IDH and AQH (p < .05) as well as the first cycle conception rate that was higher in AQH than IDH (p = .001). Metabolomic analysis quantified 56 molecules in equine seminal plasma, with 11 metabolites showing different concentrations in IDH compared to AQH (p < .05).This study provided evidence of differences in seminal plasma metabolites' concentrations between studied horse types, highlighting specific metabolomic fingerprints characterizing AQH and IDH sperm.

Citing Articles

Nuclear Magnetic Resonance (NMR) Metabolomics: Current Applications in Equine Health Assessment.

Laus F, Bazzano M, Spaterna A, Laghi L, Marchegiani A Metabolites. 2024; 14(5).

PMID: 38786746 PMC: 11123227. DOI: 10.3390/metabo14050269.

Exploring the metabolome of seminal plasma in two different horse types: Light versus draft stallions.

Bazzano M, Zhu C, Laus F, Giambattista A, Laghi L Reprod Domest Anim. 2022; 58(1):109-116.

PMID: 36151924 PMC: 10092496. DOI: 10.1111/rda.14270.

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