Seminal Plasma Amino Acid Profile in Different Breeds of Chicken: Role of Seminal Plasma on Sperm Cryoresistance

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2019 Jan 5

PMID 30608977

Citations 18

Authors

Julian Santiago-Moreno

Berenice Bernal

Serafin Perez-Cerezales

Cristina Castano

Adolfo Toledano-Diaz

Milagros C Esteso

Alfonso Gutierrez-Adan

Antonio Lopez-Sebastian

Maria G Gil

Henri Woelders

Elisabeth Blesbois

Affiliations

Soon will be listed here.

Abstract

Seminal plasma is a key biological fluid that modulates sperm function in the reproduction process. However, its role in sperm biotechnologies is scarce in poultry. The aims of the present study were to study the amino acids profile and total proteins of seminal plasma in 12 Spanish chicken breeds and to investigate the role of seminal plasma on cryoresistance of rooster sperm. To investigate the role of seminal plasma on cryoresistance, diluted pooled semen samples were cryopreserved in the presence and absence of seminal plasma. Glutamic acid was the most abundant free amino acid in seminal plasma, followed by alanine, serine, valine, and glycine. There was an influence of breed (P<0.05) on the percentage of viable sperm after freezing-thawing of samples with seminal plasma. Cluster analysis revealed that White Prat, Black Castellana, Blue Andaluza, Quail Castellana, and Red-Barred Vasca returned the best freezing-thawing response (good freezers). There was a positive correlation between seminal plasma concentrations of valine, isoleucine lysine, leucine and post thaw viability. The evaluation of fertilization capacity of frozen-thawed semen from the breeds White Prat ('good freezer') and Black-Red Andaluza ('bad freezer') showed that good freezer had higher fertility (20/68, 29.4%) compared to bad freezer breed (14/76, 18.4%), even if the difference was not significant (P = 0.08). The TUNEL assay revealed that freezing/thawing procedures in presence of seminal plasma provoked higher DNA fragmentation in most of the breeds, with a positive correlation between seminal alanine, valine, isoleucine, methionine, leucine, tyrosine, phenylalanine concentrations and DNA integrity. DNA fragmentation was lower in absence of seminal plasma and the breed effect on sperm viability was highly reduced. It is concluded that specific seminal plasma amino acids were associated with post-thaw percentage of viable sperm and DNA integrity. The removal of seminal plasma decreases the variability of the results and DNA fragmentation damages.

Citing Articles

Effects of Supplementation of Different Antioxidants to Cryopreservation Extender on the Post-Thaw Quality of Rooster Semen-A Meta-Analysis.

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Seminal plasma metabolomics analysis of differences in liquid preservation ability of boar sperm.

Song C, Chang L, Wang B, Zhang Z, Wei Y, Dou Y J Anim Sci. 2023; 101.

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Incorporation of Biotechnologies into Gene Banking Strategies to Facilitate Rapid Reconstitution of Populations.

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Origin, Selection and Current Status of the Utrerana Chicken Breed: A Review.

Plata-Casado A, Garcia-Romero C, Gonzalez-Redondo P Animals (Basel). 2023; 13(18).

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Metabolomic analysis of seminal plasma to identify goat semen freezability markers.

Xu B, Bai X, Zhang J, Li B, Zhang Y, Su R Front Vet Sci. 2023; 10:1132373.

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