Mucin Transiently Sustains Coronavirus Infectivity Through Heterogenous Changes in Phase Morphology of Evaporating Aerosol

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2022 Sep 23

PMID 36146663

Authors

Robert W Alexander

Jianghan Tian

Allen E Haddrell

Henry P Oswin

Edward Neal

Daniel A Hardy

Mara Otero-Fernandez

Jamie F S Mann

Tristan A Cogan

Adam Finn

Andrew D Davidson

Darryl J Hill

Jonathan P Reid

Affiliations

Soon will be listed here.

Abstract

Respiratory pathogens can be spread though the transmission of aerosolised expiratory secretions in the form of droplets or particulates. Understanding the fundamental aerosol parameters that govern how such pathogens survive whilst airborne is essential to understanding and developing methods of restricting their dissemination. Pathogen viability measurements made using Controlled Electrodynamic Levitation and Extraction of Bioaerosol onto Substrate (CELEBS) in tandem with a comparative kinetics electrodynamic balance (CKEDB) measurements allow for a direct comparison between viral viability and evaporation kinetics of the aerosol with a time resolution of seconds. Here, we report the airborne survival of mouse hepatitis virus (MHV) and determine a comparable loss of infectivity in the aerosol phase to our previous observations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Through the addition of clinically relevant concentrations of mucin to the bioaerosol, there is a transient mitigation of the loss of viral infectivity at 40% RH. Increased concentrations of mucin promoted heterogenous phase change during aerosol evaporation, characterised as the formation of inclusions within the host droplet. This research demonstrates the role of mucus in the aerosol phase and its influence on short-term airborne viral stability.

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