Expression of a Hydroxycinnamoyl-CoA Shikimate/Quinate Hydroxycinnamoyl Transferase 4 Gene from () Causes Excessive Elongation and Lignin Composition Changes in

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Aug 26

PMID 36012757

Authors

Di Dong

Zhuoxiong Yang

Yuan Ma

Shuwen Li

Mengdi Wang

Yinruizhi Li

Zhuocheng Liu

Chenyan Jia

Liebao Han

Yuehui Chao

Affiliations

Soon will be listed here.

Abstract

Hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT) is considered to be an essential enzyme for regulating the biosynthesis and composition of lignin. To investigate the properties and function of , the gene was cloned from with a completed coding sequence of 1284-bp in length, encoding 428 amino acids. The gene promoter has several methyl jasmonate (MeJA) response elements. According to analysis of expression patterns, it was up-regulated by MeJA, GA (Gibberellin), and SA (Salicylic acid), and down-regulated by ABA (Abscisic acid). Ectopic expression in creeping bentgrass causes excessive plant elongation. In addition, the content of G-lingnin and H-lingnin fell in transgenic plants, whereas the level of S-lingnin increased, resulting in a considerable rise in the S/G unit ratio. Analysis of the expression levels of lignin-related genes revealed that the ectopic expression of altered the expression levels of a number of genes involved in the lignin synthesis pathway. Simultaneously, MeJA, SA, GA, IAA, BR (Brassinosteroid), and other hormones were dramatically enhanced in transgenic plants relative to control plants, whereas ABA concentration was significantly decreased. Expression of impacted lignin composition and plant growth via altering the phenylpropionic acid metabolic pathway and hormone response, as revealed by transcriptome analysis. HCTs may influence plant lignin composition and plant development by altering hormone content. These findings contributed to a deeper comprehension of the lignin synthesis pathway and set the stage for further investigation and application of the gene.

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References

Weng J, Chapple C . The origin and evolution of lignin biosynthesis. New Phytol. 2010; 187(2):273-285. DOI: 10.1111/j.1469-8137.2010.03327.x. View

Dixon R, Barros J . Lignin biosynthesis: old roads revisited and new roads explored. Open Biol. 2019; 9(12):190215. PMC: 6936255. DOI: 10.1098/rsob.190215. View

Sander M, Petersen M . Distinct substrate specificities and unusual substrate flexibilities of two hydroxycinnamoyltransferases, rosmarinic acid synthase and hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyl-transferase, from Coleus blumei Benth. Planta. 2011; 233(6):1157-71. DOI: 10.1007/s00425-011-1367-2. View

Wagner A, Ralph J, Akiyama T, Flint H, Phillips L, Torr K . Exploring lignification in conifers by silencing hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyltransferase in Pinus radiata. Proc Natl Acad Sci U S A. 2007; 104(28):11856-61. PMC: 1913891. DOI: 10.1073/pnas.0701428104. View

Jang S, Hur J, Kim S, Han M, Kim S, An G . Ectopic expression of OsYAB1 causes extra stamens and carpels in rice. Plant Mol Biol. 2004; 56(1):133-43. DOI: 10.1007/s11103-004-2648-y. View

Gallego-Giraldo L, Shadle G, Shen H, Barros-Rios J, Fresquet Corrales S, Wang H . Combining enhanced biomass density with reduced lignin level for improved forage quality. Plant Biotechnol J. 2015; 14(3):895-904. PMC: 11388942. DOI: 10.1111/pbi.12439. View

Guo D, Chen F, Inoue K, Blount J, Dixon R . Downregulation of caffeic acid 3-O-methyltransferase and caffeoyl CoA 3-O-methyltransferase in transgenic alfalfa. impacts on lignin structure and implications for the biosynthesis of G and S lignin. Plant Cell. 2001; 13(1):73-88. PMC: 102215. DOI: 10.1105/tpc.13.1.73. View

Schmittgen T, Livak K . Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 2008; 3(6):1101-8. DOI: 10.1038/nprot.2008.73. View

Bonawitz N, Chapple C . The genetics of lignin biosynthesis: connecting genotype to phenotype. Annu Rev Genet. 2010; 44:337-63. DOI: 10.1146/annurev-genet-102209-163508. View

10.

Ramirez-Ahumada M, Timmermann B, Gang D . Biosynthesis of curcuminoids and gingerols in turmeric (Curcuma longa) and ginger (Zingiber officinale): identification of curcuminoid synthase and hydroxycinnamoyl-CoA thioesterases. Phytochemistry. 2006; 67(18):2017-29. DOI: 10.1016/j.phytochem.2006.06.028. View

11.

Shadle G, Chen F, Srinivasa Reddy M, Jackson L, Nakashima J, Dixon R . Down-regulation of hydroxycinnamoyl CoA: shikimate hydroxycinnamoyl transferase in transgenic alfalfa affects lignification, development and forage quality. Phytochemistry. 2007; 68(11):1521-9. DOI: 10.1016/j.phytochem.2007.03.022. View

12.

Luo H, Hu Q, Nelson K, Longo C, Kausch A, Chandlee J . Agrobacterium tumefaciens-mediated creeping bentgrass (Agrostis stolonifera L.) transformation using phosphinothricin selection results in a high frequency of single-copy transgene integration. Plant Cell Rep. 2003; 22(9):645-52. DOI: 10.1007/s00299-003-0734-2. View

13.

Hoffmann L, Besseau S, Geoffroy P, Ritzenthaler C, Meyer D, Lapierre C . Silencing of hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyltransferase affects phenylpropanoid biosynthesis. Plant Cell. 2004; 16(6):1446-65. PMC: 490038. DOI: 10.1105/tpc.020297. View

14.

Boerjan W, Ralph J, Baucher M . Lignin biosynthesis. Annu Rev Plant Biol. 2003; 54:519-46. DOI: 10.1146/annurev.arplant.54.031902.134938. View

15.

Kumar S, Stecher G, Li M, Knyaz C, Tamura K . MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018; 35(6):1547-1549. PMC: 5967553. DOI: 10.1093/molbev/msy096. View

16.

Clough S, Bent A . Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1999; 16(6):735-43. DOI: 10.1046/j.1365-313x.1998.00343.x. View

17.

Anterola A, Lewis N . Trends in lignin modification: a comprehensive analysis of the effects of genetic manipulations/mutations on lignification and vascular integrity. Phytochemistry. 2002; 61(3):221-94. DOI: 10.1016/s0031-9422(02)00211-x. View

18.

Hoffmann L, Maury S, Martz F, Geoffroy P, Legrand M . Purification, cloning, and properties of an acyltransferase controlling shikimate and quinate ester intermediates in phenylpropanoid metabolism. J Biol Chem. 2002; 278(1):95-103. DOI: 10.1074/jbc.M209362200. View

19.

Vanholme R, Demedts B, Morreel K, Ralph J, Boerjan W . Lignin biosynthesis and structure. Plant Physiol. 2010; 153(3):895-905. PMC: 2899938. DOI: 10.1104/pp.110.155119. View

20.

Dong D, Zhao Y, Teng K, Tan P, Liu Z, Yang Z . Expression of , a Phytoene Synthase Gene from Affects Plant Height and Photosynthetic Pigment Contents. Plants (Basel). 2022; 11(3). PMC: 8840084. DOI: 10.3390/plants11030395. View