Agrobacterium Tumefaciens-mediated Creeping Bentgrass (Agrostis Stolonifera L.) Transformation Using Phosphinothricin Selection Results in a High Frequency of Single-copy Transgene Integration

Overview

Journal Plant Cell Rep

Publisher Springer

Date 2003 Nov 15

PMID 14615907

Citations 27

Authors

H Luo

Q Hu

K Nelson

C Longo

A P Kausch

J M Chandlee

J K Wipff

C R Fricker

Affiliations

Soon will be listed here.

Abstract

Genetic transformation of creeping bentgrass mediated by Agrobacterium tumefaciens has been achieved. Embryogenic callus initiated from seeds (cv. Penn-A-4) was infected with an A. tumefaciens strain (LBA4404) harboring a super-binary vector that contained an herbicide-resistant bar gene driven either by the CaMV 35S promoter or a rice ubiquitin promoter. Plants were regenerated from 219 independent transformation events. The overall stable transformation efficiency ranged from 18% to 45%. Southern blot and genetic analysis confirmed transgene integration in the creeping bentgrass genome and normal transmission and stable expression of the transgene in the T1 generation. All independent transformation events carried one to three copies of the transgene, and a majority (60-65%) contained only a single copy of the foreign gene with no apparent rearrangements. We report here the successful use of Agrobacterium for the large-scale production of transgenic creeping bentgrass plants with a high frequency of a single-copy transgene insertion that exhibit stable inheritance patterns.

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