Analysis of Single-Cell Transcriptome Data in Drosophila

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2022 Aug 18

PMID 35980574

Authors

Schayan Yousefian

Maria Jelena Musillo

Josephine Bageritz

Affiliations

Soon will be listed here.

Abstract

The fly Drosophila is a versatile model organism that has led to fascinating biological discoveries. In the past few years, Drosophila researchers have used single-cell RNA-sequencing (scRNA-seq) to gain insights into the cellular composition, and developmental processes of various tissues and organs. Given the success of single-cell technologies a variety of computational tools and software packages were developed to enable and facilitate the analysis of scRNA-seq data. In this book chapter we want to give guidance on analyzing droplet-based scRNA-seq data from Drosophila. We will initially describe the preprocessing commonly done for Drosophila, point out possible downstream analyses, and finally highlight computational methods developed using Drosophila scRNA-seq data.

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