Reduction in the Migration Activity of Microglia Treated with Silica-Coated Magnetic Nanoparticles and Their Recovery Using Citrate

Overview

Journal Cells

Publisher MDPI

Specialties Biochemistry
Biophysics
Cell Biology
Molecular Biology

Date 2022 Aug 12

PMID 35954236

Authors

Tae Hwan Shin

Da Yeon Lee

Yong Eun Jang

Do Hyeon Kwon

Ji Su Hwang

Seok Gi Kim

Chan Seo

Man Jeong Paik

Ju Yeon Lee

Jin Young Kim

Seokho Park

Sung-E Choi

Shaherin Basith

Myeong Ok Kim

Gwang Lee

Affiliations

Soon will be listed here.

Abstract

Nanoparticles have garnered significant interest in neurological research in recent years owing to their efficient penetration of the blood-brain barrier (BBB). However, significant concerns are associated with their harmful effects, including those related to the immune response mediated by microglia, the resident immune cells in the brain, which are exposed to nanoparticles. We analysed the cytotoxic effects of silica-coated magnetic nanoparticles containing rhodamine B isothiocyanate dye [MNPs@SiO(RITC)] in a BV2 microglial cell line using systems toxicological analysis. We performed the invasion assay and the exocytosis assay and transcriptomics, proteomics, metabolomics, and integrated triple-omics analysis, generating a single network using a machine learning algorithm. The results highlight alteration in the mechanisms of the nanotoxic effects of nanoparticles using integrated omics analysis.

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