Human Umbilical Cord Wharton Jelly Cells Treatment Prevents Osteoporosis Induced by D-Galactose

Overview

Journal Int J Clin Pract

Publisher Wiley

Specialty General Medicine

Date 2022 Aug 8

PMID 35936064

Authors

Qiang Wang

Zhiqiang Gao

Kai Guo

Jiawei Lu

Feng Wang

Tongde Wu

Yufeng Huang

Desheng Wu

Affiliations

Soon will be listed here.

Abstract

Methods: Sixteen male mice were randomly divided into 4 groups: control (ordinary feeding), D-gal (D-galactose) group, D-gal + MSC (human umbilical cord Wharton jelly cells), and D-gal + MSC-TNF groups. Except for the control group (fed with same amount of saline solution), other mice received gastric feeding of 250 mg/kg D-galactose every day for 8 weeks. TNF (10 ng/mL for 24 h) cocultured or noncocultured HUCWJCs (5 × 10) were suspended in 0.1 ml of sterile PBS and injected into tail veins every other week in D-gal + MSC-TNF and D-gal + MSC groups, respectively, and only 0.1 ml of sterile PBS for control and D-gal groups. The bone mass was detected by qPCR, ELISA, microcomputed tomography (CT), and hematoxylin-eosin staining. Proliferation, apoptosis, and differentiation of periosteal-derived osteoblasts (POB) were assessed. Transwell assay and scratch healing were performed to detect POB migration and invasion ability. The effect of HUCWJCs on POB signaling pathway expression was evaluated by immunoblotting.

Results: The malondialdehyde (MDA) in serum was higher and superoxide dismutase (SOD) was lower in the D-gal group compared to the other groups ( < 0.05). Mice in D-gal group showed significantly decreased bone mass when compared to the control group, while HUCWJCs treatment partially rescued the phenotype, as demonstrated by CT and histology ( < 0.05). Mechanically, HUCWJCs treatment partially promoted proliferation and migration and decreased apoptosis of POB induced by oxidative stress via activating the mitogen-activated protein kinase (MAPK) signaling pathway.

Conclusion: HUCWJCs can prevent the progression of osteoporosis by inhibiting oxidative stress, which may act by regulating osteoblasts fate through the MAPK signaling pathway.

References

Chen W, Tsai Y, Shibu M, Shen C, Chang-Lee S, Chen R . Exercise training augments Sirt1-signaling and attenuates cardiac inflammation in D-galactose induced-aging rats. Aging (Albany NY). 2018; 10(12):4166-4174. PMC: 6326662. DOI: 10.18632/aging.101714. View

Li W, Liu Y, Zhang P, Tang Y, Zhou M, Jiang W . Tissue-Engineered Bone Immobilized with Human Adipose Stem Cells-Derived Exosomes Promotes Bone Regeneration. ACS Appl Mater Interfaces. 2018; 10(6):5240-5254. DOI: 10.1021/acsami.7b17620. View

Yahao G, Xinjia W . The Role and Mechanism of Exosomes from Umbilical Cord Mesenchymal Stem Cells in Inducing Osteogenesis and Preventing Osteoporosis. Cell Transplant. 2021; 30:9636897211057465. PMC: 8647230. DOI: 10.1177/09636897211057465. View

Cui X, Zuo P, Zhang Q, Li X, Hu Y, Long J . Chronic systemic D-galactose exposure induces memory loss, neurodegeneration, and oxidative damage in mice: protective effects of R-alpha-lipoic acid. J Neurosci Res. 2006; 83(8):1584-90. DOI: 10.1002/jnr.20845. View

Zhang Y, Andrukhov O, Berner S, Matejka M, Wieland M, Rausch-Fan X . Osteogenic properties of hydrophilic and hydrophobic titanium surfaces evaluated with osteoblast-like cells (MG63) in coculture with human umbilical vein endothelial cells (HUVEC). Dent Mater. 2010; 26(11):1043-51. DOI: 10.1016/j.dental.2010.07.003. View

Liu C, Zhang H, Tang X, Feng R, Yao G, Chen W . Mesenchymal Stem Cells Promote the Osteogenesis in Collagen-Induced Arthritic Mice through the Inhibition of TNF-. Stem Cells Int. 2018; 2018:4069032. PMC: 5964571. DOI: 10.1155/2018/4069032. View

Hu Y, Zhang Y, Ni C, Chen C, Rao S, Yin H . Human umbilical cord mesenchymal stromal cells-derived extracellular vesicles exert potent bone protective effects by CLEC11A-mediated regulation of bone metabolism. Theranostics. 2020; 10(5):2293-2308. PMC: 7019162. DOI: 10.7150/thno.39238. View

Rochefort G, Pallu S, Benhamou C . Osteocyte: the unrecognized side of bone tissue. Osteoporos Int. 2010; 21(9):1457-69. DOI: 10.1007/s00198-010-1194-5. View

Zhao P, Xiao L, Peng J, Qian Y, Huang C . Exosomes derived from bone marrow mesenchymal stem cells improve osteoporosis through promoting osteoblast proliferation via MAPK pathway. Eur Rev Med Pharmacol Sci. 2018; 22(12):3962-3970. DOI: 10.26355/eurrev_201806_15280. View

10.

Hu L, Yin C, Zhao F, Ali A, Ma J, Qian A . Mesenchymal Stem Cells: Cell Fate Decision to Osteoblast or Adipocyte and Application in Osteoporosis Treatment. Int J Mol Sci. 2018; 19(2). PMC: 5855582. DOI: 10.3390/ijms19020360. View

11.

Wang S, Feng W, Liu J, Wang X, Zhong L, Lv C . Alginate oligosaccharide alleviates senile osteoporosis via the RANKL-RANK pathway in D-galactose-induced C57BL/6J mice. Chem Biol Drug Des. 2021; 99(1):46-55. PMC: 9544009. DOI: 10.1111/cbdd.13904. View

12.

Raehtz S, Bierhalter H, Schoenherr D, Parameswaran N, McCabe L . Estrogen Deficiency Exacerbates Type 1 Diabetes-Induced Bone TNF-α Expression and Osteoporosis in Female Mice. Endocrinology. 2017; 158(7):2086-2101. PMC: 5505215. DOI: 10.1210/en.2016-1821. View

13.

Ji M, Su X, Liu J, Zhao Y, Li Z, Xu X . Comparison of naturally aging and D-galactose induced aging model in beagle dogs. Exp Ther Med. 2017; 14(6):5881-5888. PMC: 5740571. DOI: 10.3892/etm.2017.5327. View

14.

Liu M, Wang R, Xing J, Tang Y . Atractylenolide inhibits apoptosis and oxidative stress of HTR-8/SVneo cells by activating MAPK/ERK signalling in preeclampsia. Phytomedicine. 2021; 93:153773. DOI: 10.1016/j.phymed.2021.153773. View

15.

Jilka R, Noble B, Weinstein R . Osteocyte apoptosis. Bone. 2012; 54(2):264-71. PMC: 3624050. DOI: 10.1016/j.bone.2012.11.038. View

16.

Ezzat S, L Louka M, Zakaria Z, Nagaty M, Metwaly R . Autophagy in osteoporosis: Relation to oxidative stress. J Cell Biochem. 2018; 120(2):2560-2568. DOI: 10.1002/jcb.27552. View

17.

Zhou Q, Zhu L, Zhang D, Li N, Li Q, Dai P . Oxidative Stress-Related Biomarkers in Postmenopausal Osteoporosis: A Systematic Review and Meta-Analyses. Dis Markers. 2016; 2016:7067984. PMC: 4995322. DOI: 10.1155/2016/7067984. View

18.

Torres M, Wanionok N, McCarthy A, Morel G, Fernandez J . Systemic oxidative stress in old rats is associated with both osteoporosis and cognitive impairment. Exp Gerontol. 2021; 156:111596. DOI: 10.1016/j.exger.2021.111596. View

19.

Rebelatto C, Aguiar A, Moretao M, Senegaglia A, Hansen P, Barchiki F . Dissimilar differentiation of mesenchymal stem cells from bone marrow, umbilical cord blood, and adipose tissue. Exp Biol Med (Maywood). 2008; 233(7):901-13. DOI: 10.3181/0712-RM-356. View

20.

Ilic D, Miere C, Lazic E . Umbilical cord blood stem cells: clinical trials in non-hematological disorders. Br Med Bull. 2012; 102:43-57. DOI: 10.1093/bmb/lds008. View