CircRPPH1_025 Overexpression Promotes Migration and Invasion of Glioblastoma Multiforme

Overview

Journal Dis Markers

Publisher Wiley

Specialty Biochemistry

Date 2022 Aug 5

PMID 35928926

Authors

Lixiong Xue

Huahui Chen

Xiaolong Wang

Li Han

Yifan Liu

Xinmin Ding

Affiliations

Soon will be listed here.

Abstract

Objective: To study the effect of circ_0000512 (circRPPH1_025) on the tumorigensis and development of glioblastoma and its molecular mechanism.

Methods: The expression levels of circ_0000512 in normal astrocytes (NHA) and human glioblastoma cell lines (U87, U251, and A172) and the expression levels of circ_0000512 and linear RNA RPPH1 in U87 cells after RNase R treatment were detected by qRT-PCR. The effects of circ_0000512 knockdown or overexpression on the proliferation, migration, invasion, and epithelial-mesenchymal transition of U87 cells were detected by CCK-8 assay, cell colony formation assay, transwell invasion assay, wound healing assay, and western blot.

Results: The expression of circ_0000512 was upregulated in glioblastoma cells, and the overexpression of circ_0000512 was beneficial to the proliferation, migration, invasion, and epithelial-mesenchymal transition of U87 cells, while knockdown of circ_0000512 showed the opposite results.

Conclusion: circ_0000512 can be used as a potential target for early diagnosis and targeted therapy of glioblastoma multiforme.

Citing Articles

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References

Cloughesy T, Cavenee W, Mischel P . Glioblastoma: from molecular pathology to targeted treatment. Annu Rev Pathol. 2013; 9:1-25. DOI: 10.1146/annurev-pathol-011110-130324. View

Liu J, Dai X, Guo X, Cheng A, Mac S, Wang Z . Circ-OXCT1 Suppresses Gastric Cancer EMT and Metastasis by Attenuating TGF-β Pathway Through the Circ-OXCT1/miR-136/SMAD4 Axis. Onco Targets Ther. 2020; 13:3987-3998. PMC: 7236241. DOI: 10.2147/OTT.S239789. View

Wang L, Wu H, Chu F, Zhang L, Xiao X . Knockdown of circ_0000512 Inhibits Cell Proliferation and Promotes Apoptosis in Colorectal Cancer by Regulating miR-296-5p/RUNX1 Axis. Onco Targets Ther. 2020; 13:7357-7368. PMC: 7419622. DOI: 10.2147/OTT.S250495. View

Georgescu M, Olar A . Genetic and histologic spatiotemporal evolution of recurrent, multifocal, multicentric and metastatic glioblastoma. Acta Neuropathol Commun. 2020; 8(1):10. PMC: 6998196. DOI: 10.1186/s40478-020-0889-x. View

Ma D, Zheng B, Suzuki T, Zhang R, Jiang C, Bai D . Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm. Circ Res. 2017; 120(5):799-815. DOI: 10.1161/CIRCRESAHA.116.310367. View

Zhou J, Chen K, Hu S, Dong J, Wang H, Su X . Up-regulation of circular RNA hsa_circ_01844 induces apoptosis and suppresses proliferation and migration of glioblastoma cells. Chin Med J (Engl). 2020; 134(1):81-87. PMC: 7862802. DOI: 10.1097/CM9.0000000000000979. View

Yan Y, Zhang R, Zhang X, Zhang A, Zhang Y, Bu X . RNA-Seq profiling of circular RNAs and potential function of hsa_circ_0002360 in human lung adenocarcinom. Am J Transl Res. 2019; 11(1):160-175. PMC: 6357307. View

Gheldof A, Berx G . Cadherins and epithelial-to-mesenchymal transition. Prog Mol Biol Transl Sci. 2013; 116:317-36. DOI: 10.1016/B978-0-12-394311-8.00014-5. View

Delgado-Lopez P, Corrales-Garcia E . Survival in glioblastoma: a review on the impact of treatment modalities. Clin Transl Oncol. 2016; 18(11):1062-1071. DOI: 10.1007/s12094-016-1497-x. View

10.

Lv T, Miao Y, Xu T, Sun W, Sang Y, Jia F . Circ-EPB41L5 regulates the host gene via sponging miR-19a to repress glioblastoma tumorigenesis. Aging (Albany NY). 2020; 12(1):318-339. PMC: 6977680. DOI: 10.18632/aging.102617. View

11.

Zhu F, Cheng C, Qin H, Wang H, Yu H . A novel circular RNA circENTPD7 contributes to glioblastoma progression by targeting ROS1. Cancer Cell Int. 2020; 20:118. PMC: 7147020. DOI: 10.1186/s12935-020-01208-9. View

12.

Zhao J, Zhang L, Dong X, Liu L, Huo L, Chen H . High Expression of Vimentin is Associated With Progression and a Poor Outcome in Glioblastoma. Appl Immunohistochem Mol Morphol. 2016; 26(5):337-344. DOI: 10.1097/PAI.0000000000000420. View

13.

Aliotta E, Nourzadeh H, Batchala P, Schiff D, Lopes M, Druzgal J . Molecular Subtype Classification in Lower-Grade Glioma with Accelerated DTI. AJNR Am J Neuroradiol. 2019; 40(9):1458-1463. PMC: 7048441. DOI: 10.3174/ajnr.A6162. View

14.

Liu B, Dong H, Lin X, Yang X, Yue X, Yang J . RND3 promotes Snail 1 protein degradation and inhibits glioblastoma cell migration and invasion. Oncotarget. 2016; 7(50):82411-82423. PMC: 5347701. DOI: 10.18632/oncotarget.12396. View

15.

Zhu J, Ye J, Zhang L, Xia L, Hu H, Jiang H . Differential Expression of Circular RNAs in Glioblastoma Multiforme and Its Correlation with Prognosis. Transl Oncol. 2017; 10(2):271-279. PMC: 5328755. DOI: 10.1016/j.tranon.2016.12.006. View

16.

Mittal V . Epithelial Mesenchymal Transition in Tumor Metastasis. Annu Rev Pathol. 2018; 13:395-412. DOI: 10.1146/annurev-pathol-020117-043854. View

17.

Xia X, Li X, Li F, Wu X, Zhang M, Zhou H . A novel tumor suppressor protein encoded by circular AKT3 RNA inhibits glioblastoma tumorigenicity by competing with active phosphoinositide-dependent Kinase-1. Mol Cancer. 2019; 18(1):131. PMC: 6716823. DOI: 10.1186/s12943-019-1056-5. View

18.

Kulcheski F, Christoff A, Margis R . Circular RNAs are miRNA sponges and can be used as a new class of biomarker. J Biotechnol. 2016; 238:42-51. DOI: 10.1016/j.jbiotec.2016.09.011. View

19.

Xia W, Qiu M, Chen R, Wang S, Leng X, Wang J . Circular RNA has_circ_0067934 is upregulated in esophageal squamous cell carcinoma and promoted proliferation. Sci Rep. 2016; 6:35576. PMC: 5067712. DOI: 10.1038/srep35576. View

20.

Yuan F, Sun Q, Xu Y, Zhang H, Deng G, Yang J . Hsa_circ_0072309 inhibits proliferation and invasion of glioblastoma. Pathol Res Pract. 2021; 222:153433. DOI: 10.1016/j.prp.2021.153433. View