RNA-Seq Profiling of Circular RNAs and Potential Function of Hsa_circ_0002360 in Human Lung Adenocarcinom

Overview

Journal Am J Transl Res

Specialty General Medicine

Date 2019 Feb 22

PMID 30787976

Citations 21

Authors

Yulan Yan

Riting Zhang

Xuanfeng Zhang

Anwei Zhang

Yao Zhang

Xuefeng Bu

Affiliations

Soon will be listed here.

Abstract

CircRNAs have been identified play a key role in various different types of cancer. However, their role in lung adenocarcinoma remains unclear. In this study, we explored the specific circular transcriptome and characterized the circRNA expression profiles of five paired lung adenocarcinoma (LAC) tissues relative to adjacent normal tissues from LAC patients using next-generation sequencing (NGS). To illuminate circRNAs function, their gene targets were initially predicted before using, Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses to further analyse the associated significant cell signalling pathways and functions. The potential interactions between circRNA-miRNA-mRNA were also investigated. Additionally, qRT-PCR assay, Western blot and Immunohistochemistry were performed to validate the differential expression of circRNA, microRNA and mRNA in the LAC group in comparison to the control group. Two-hundred-eighty-five dysregulated circular transcripts were found in LAC tissues, among which 102 and 183 were either up or down regulated, respectively. Our biological analysis suggested that the host genes of differentially expressed circRNAs targeted to cancer-related processes and mechanisms. The interaction maps of the circRNA-miRNA-target gene were constructed using Cytoscope. In further study, hsa_circ_0002360 was found to be the most significantly overexpressed circRNA in LAC tissues by interacting with miRNA and its corresponding mRNA. Our results showed that hsa_circ_0002360 was aberrantly and abundantly expressed and implicated in the development of LAC, suggesting a valuable therapeutic target for LAC treatment.

Citing Articles

Has_circ_0002360 promotes the progression of lung adenocarcinoma by activating miR-762 and regulating PODXL expression.

Yan Y, Zhang Y, He Y, Bu X Transl Cancer Res. 2024; 13(8):4172-4186.

PMID: 39262484 PMC: 11384312. DOI: 10.21037/tcr-24-279.

Circular RNAs in lung cancer: implications for preventing therapeutic resistance.

Liu W, Sun Y, Huo Y, Zhang L, Zhang N, Yang M EBioMedicine. 2024; 107:105309.

PMID: 39191172 PMC: 11445705. DOI: 10.1016/j.ebiom.2024.105309.

Identification of Circular RNAs as Biomarker Candidates in Lung Cancer Treatment.

Apaydin B, Mert U, Asadi M, Muftuoglu C, Caner A Asian Pac J Cancer Prev. 2024; 25(6):2147-2157.

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CircRUNX1 drives the malignant phenotypes of lung adenocarcinoma through mediating the miR-5195-3p/HMGB3 network.

Wang Y, Li H, Chen W, Huang X, Fan R, Xu M Gen Thorac Cardiovasc Surg. 2023; 72(3):164-175.

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Curcumin suppresses lung cancer progression via circRUNX1 mediated miR-760/RAB3D axis.

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References

Sanger H, KLOTZ G, Riesner D, Gross H, KLEINSCHMIDT A . Viroids are single-stranded covalently closed circular RNA molecules existing as highly base-paired rod-like structures. Proc Natl Acad Sci U S A. 1976; 73(11):3852-6. PMC: 431239. DOI: 10.1073/pnas.73.11.3852. View

Goto S, Okuno Y, Hattori M, Nishioka T, Kanehisa M . LIGAND: database of chemical compounds and reactions in biological pathways. Nucleic Acids Res. 2001; 30(1):402-4. PMC: 99090. DOI: 10.1093/nar/30.1.402. View

Dennis Jr G, Sherman B, Hosack D, Yang J, Gao W, Lane H . DAVID: Database for Annotation, Visualization, and Integrated Discovery. Genome Biol. 2003; 4(5):P3. View

Wang S, Robertson G, Zhu J . A novel human homologue of Drosophila polycomblike gene is up-regulated in multiple cancers. Gene. 2004; 343(1):69-78. DOI: 10.1016/j.gene.2004.09.006. View

Bozcuk H, Artac M, Ozdogan M, Savas B . Does maintenance/consolidation chemotherapy have a role in the management of small cell lung cancer (SCLC)? A metaanalysis of the published controlled trials. Cancer. 2005; 104(12):2650-7. DOI: 10.1002/cncr.21540. View

Garzon R, Fabbri M, Cimmino A, Calin G, Croce C . MicroRNA expression and function in cancer. Trends Mol Med. 2006; 12(12):580-7. DOI: 10.1016/j.molmed.2006.10.006. View

Bailey C, Kelly P, Casey P . Activation of Rap1 promotes prostate cancer metastasis. Cancer Res. 2009; 69(12):4962-8. PMC: 4195850. DOI: 10.1158/0008-5472.CAN-08-4269. View

Wang L, Feng Z, Wang X, Wang X, Zhang X . DEGseq: an R package for identifying differentially expressed genes from RNA-seq data. Bioinformatics. 2009; 26(1):136-8. DOI: 10.1093/bioinformatics/btp612. View

Jiang L, Huang Q, Zhang S, Zhang Q, Chang J, Qiu X . Hsa-miR-125a-3p and hsa-miR-125a-5p are downregulated in non-small cell lung cancer and have inverse effects on invasion and migration of lung cancer cells. BMC Cancer. 2010; 10:318. PMC: 2903529. DOI: 10.1186/1471-2407-10-318. View

10.

Langer C, Besse B, Gualberto A, Brambilla E, Soria J . The evolving role of histology in the management of advanced non-small-cell lung cancer. J Clin Oncol. 2010; 28(36):5311-20. DOI: 10.1200/JCO.2010.28.8126. View

11.

Vasioukhin V . Adherens junctions and cancer. Subcell Biochem. 2012; 60:379-414. DOI: 10.1007/978-94-007-4186-7_16. View

12.

Hansen T, Jensen T, Clausen B, Bramsen J, Finsen B, Damgaard C . Natural RNA circles function as efficient microRNA sponges. Nature. 2013; 495(7441):384-8. DOI: 10.1038/nature11993. View

13.

Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A . Circular RNAs are a large class of animal RNAs with regulatory potency. Nature. 2013; 495(7441):333-8. DOI: 10.1038/nature11928. View

14.

Zhang Y, Zhang X, Chen T, Xiang J, Yin Q, Xing Y . Circular intronic long noncoding RNAs. Mol Cell. 2013; 51(6):792-806. DOI: 10.1016/j.molcel.2013.08.017. View

15.

Li G, Warden C, Zou Z, Neman J, Krueger J, Jain A . Altered expression of polycomb group genes in glioblastoma multiforme. PLoS One. 2013; 8(11):e80970. PMC: 3829908. DOI: 10.1371/journal.pone.0080970. View

16.

Tay Y, Rinn J, Pandolfi P . The multilayered complexity of ceRNA crosstalk and competition. Nature. 2014; 505(7483):344-52. PMC: 4113481. DOI: 10.1038/nature12986. View

17.

Ma X, Jia Y, Zu S, Li R, Jia Y, Zhao Y . α5 Nicotinic acetylcholine receptor mediates nicotine-induced HIF-1α and VEGF expression in non-small cell lung cancer. Toxicol Appl Pharmacol. 2014; 278(2):172-9. DOI: 10.1016/j.taap.2014.04.023. View

18.

Anders S, Pyl P, Huber W . HTSeq--a Python framework to work with high-throughput sequencing data. Bioinformatics. 2014; 31(2):166-9. PMC: 4287950. DOI: 10.1093/bioinformatics/btu638. View

19.

Bahn J, Zhang Q, Li F, Chan T, Lin X, Kim Y . The landscape of microRNA, Piwi-interacting RNA, and circular RNA in human saliva. Clin Chem. 2014; 61(1):221-30. PMC: 4332885. DOI: 10.1373/clinchem.2014.230433. View

20.

Wang Y, Wang Z . Efficient backsplicing produces translatable circular mRNAs. RNA. 2014; 21(2):172-9. PMC: 4338345. DOI: 10.1261/rna.048272.114. View