Defining the Proximal Interaction Networks of Arf GTPases Reveals a Mechanism for the Regulation of PLD1 and PI4KB

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2022 Jul 18

PMID 35844135

Authors

Fu-Long Li

Zhengming Wu

Yong-Qi Gao

Forrest Z Bowling

J Matthew Franklin

Chongze Hu

Raymond T Suhandynata

Michael A Frohman

Michael V Airola

Huilin Zhou

Kun-Liang Guan

Affiliations

Soon will be listed here.

Abstract

The Arf GTPase family is involved in a wide range of cellular regulation including membrane trafficking and organelle-structure assembly. Here, we have generated a proximity interaction network for the Arf family using the miniTurboID approach combined with TMT-based quantitative mass spectrometry. Our interactome confirmed known interactions and identified many novel interactors that provide leads for defining Arf pathway cell biological functions. We explored the unexpected finding that phospholipase D1 (PLD1) preferentially interacts with two closely related but poorly studied Arf family GTPases, ARL11 and ARL14, showing that PLD1 is activated by ARL11/14 and may recruit these GTPases to membrane vesicles, and that PLD1 and ARL11 collaborate to promote macrophage phagocytosis. Moreover, ARL5A and ARL5B were found to interact with and recruit phosphatidylinositol 4-kinase beta (PI4KB) at trans-Golgi, thus promoting PI4KB's function in PI4P synthesis and protein secretion.

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