Optimization and Use of Near Infrared Imaging to Guide Lymph Node Collection in Rhesus Macaques (Macaca Mulatta)

Overview

Journal J Med Primatol

Publisher Wiley

Date 2022 Jul 16

PMID 35841132

Authors

Jeremy V Smedley

Rachele M Bochart

Miranda Fischer

Heidi Funderburgh

Vanessa Kelly

Hugh Crank

Kim Armantrout

Oriene Shiel

Mitchell Robertson-LeVay

Nikki Sternberger

Brian Schmaling

Sheila Roberts

Vicki Sekiguchi

Michael Reusz

Tiah Schwartz

Kimberly A Meyer

Gabriela Webb

Roxanne M Gilbride

Nicholas Dambrauskas

Daniela Andrade

Matthew Wood

Caralyn Labriola

Michael Axthelm

Nina Derby

Ben Varco-Merth

Yoshinori Fukazawa

Scott Hansen

Jonah B Sacha

Donald L Sodora

D Noah Sather

Affiliations

Soon will be listed here.

Abstract

Background: Identification of lymph nodes (LNs) draining a specific site or in obese macaques can be challenging.

Methods: Indocyanine Green (ICG) was administered intradermal (ID), intramuscular, in the oral mucosa, or subserosal in the colon followed by Near Infrared (NIR) imaging.

Results: After optimization to maximize LN identification, intradermal ICG was successful in identifying 50-100% of the axillary/inguinal LN at a site. Using NIR, collection of peripheral and mesenteric LNs in obese macaques was 100% successful after traditional methods failed. Additionally, guided collection of LNs draining the site of intraepithelial or intramuscular immunization demonstrated significantly increased numbers of T follicular helper (Tfh) cells in germinal centers of draining compared to nondraining LNs.

Conclusion: These imaging techniques optimize our ability to evaluate immune changes within LNs over time, even in obese macaques. This approach allows for targeted serial biopsies that permit confidence that draining LNs are being harvested throughout the study.

Citing Articles

Editorial: Preclinical macaque models of viral diseases.

Smedley J Front Immunol. 2023; 14:1331774.

PMID: 38022655 PMC: 10666555. DOI: 10.3389/fimmu.2023.1331774.

Optimization and use of near infrared imaging to guide lymph node collection in rhesus macaques (Macaca mulatta).

Smedley J, Bochart R, Fischer M, Funderburgh H, Kelly V, Crank H J Med Primatol. 2022; 51(5):270-277.

PMID: 35841132 PMC: 9474636. DOI: 10.1111/jmp.12605.

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