Antibody-Based Affinity Capture Combined with LC-MS Analysis for Identification of COVID-19 Disease Serum Biomarkers

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2022 Jul 15

PMID 35838961

Authors

Paul C Guest

Hassan Rahmoune

Affiliations

Soon will be listed here.

Abstract

Blood serum or plasma proteins are potentially useful in COVID-19 research as biomarkers for risk prediction, diagnosis, stratification, and treatment monitoring. However, serum protein-based biomarker identification and validation is complicated due to the wide concentration range of these proteins, which spans more than ten orders of magnitude. Here we present a combined affinity purification-liquid chromatography mass spectrometry approach which allows identification and quantitation of the most abundant serum proteins along with the nonspecifically bound and interaction proteins. This led to the reproducible identification of more than 100 proteins that were not specifically targeted by the affinity column. Many of these have already been implicated in COVID-19 disease.

References

Rodriguez Y, Novelli L, Rojas M, De Santis M, Acosta-Ampudia Y, Monsalve D . Autoinflammatory and autoimmune conditions at the crossroad of COVID-19. J Autoimmun. 2020; 114:102506. PMC: 7296326. DOI: 10.1016/j.jaut.2020.102506. View

Vardhana S, Wolchok J . The many faces of the anti-COVID immune response. J Exp Med. 2020; 217(6). PMC: 7191310. DOI: 10.1084/jem.20200678. View

Zhou Y, Zhang J, Wang D, Wang D, Guan W, Qin J . Profiling of the immune repertoire in COVID-19 patients with mild, severe, convalescent, or retesting-positive status. J Autoimmun. 2021; 118:102596. PMC: 7837046. DOI: 10.1016/j.jaut.2021.102596. View

Gando S, Wada T . Thromboplasminflammation in COVID-19 Coagulopathy: Three Viewpoints for Diagnostic and Therapeutic Strategies. Front Immunol. 2021; 12:649122. PMC: 8226122. DOI: 10.3389/fimmu.2021.649122. View

Semeraro N, Colucci M . The Prothrombotic State Associated with SARS-CoV-2 Infection: Pathophysiological Aspects. Mediterr J Hematol Infect Dis. 2021; 13(1):e2021045. PMC: 8265369. DOI: 10.4084/MJHID.2021.045. View

Pascolo S . Synthetic Messenger RNA-Based Vaccines: from Scorn to Hype. Viruses. 2021; 13(2). PMC: 7916233. DOI: 10.3390/v13020270. View

Akbar M, Pranata R, Wibowo A, Lim M, Sihite T, Martha J . The association between serum prealbumin and poor outcome in COVID-19 - Systematic review and meta-analysis. Eur Rev Med Pharmacol Sci. 2021; 25(10):3879-3885. DOI: 10.26355/eurrev_202105_25955. View

Liu K, Yang T, Peng X, Lv S, Ye X, Zhao T . A systematic meta-analysis of immune signatures in patients with COVID-19. Rev Med Virol. 2021; 31(4):e2195. PMC: 7744845. DOI: 10.1002/rmv.2195. View

Pang N, Pang A, Chow V, Wang D . Understanding neutralising antibodies against SARS-CoV-2 and their implications in clinical practice. Mil Med Res. 2021; 8(1):47. PMC: 8405719. DOI: 10.1186/s40779-021-00342-3. View

10.

Merle N, Church S, Fremeaux-Bacchi V, Roumenina L . Complement System Part I - Molecular Mechanisms of Activation and Regulation. Front Immunol. 2015; 6:262. PMC: 4451739. DOI: 10.3389/fimmu.2015.00262. View

11.

Wu Y, Huang X, Sun J, Xie T, Lei Y, Muhammad J . Clinical Characteristics and Immune Injury Mechanisms in 71 Patients with COVID-19. mSphere. 2020; 5(4). PMC: 7364211. DOI: 10.1128/mSphere.00362-20. View

12.

Tang Y, Sun J, Pan H, Yao F, Yuan Y, Zeng M . Aberrant cytokine expression in COVID-19 patients: Associations between cytokines and disease severity. Cytokine. 2021; 143:155523. PMC: 8011640. DOI: 10.1016/j.cyto.2021.155523. View

13.

Pfister F, Vonbrunn E, Ries T, Jack H, Uberla K, Lochnit G . Complement Activation in Kidneys of Patients With COVID-19. Front Immunol. 2021; 11:594849. PMC: 7878379. DOI: 10.3389/fimmu.2020.594849. View

14.

Jamaly S, Tsokos M, Bhargava R, Brook O, Hecht J, Abdi R . Complement activation and increased expression of Syk, mucin-1 and CaMK4 in kidneys of patients with COVID-19. Clin Immunol. 2021; 229:108795. PMC: 8270746. DOI: 10.1016/j.clim.2021.108795. View

15.

Messner C, Demichev V, Wendisch D, Michalick L, White M, Freiwald A . Ultra-High-Throughput Clinical Proteomics Reveals Classifiers of COVID-19 Infection. Cell Syst. 2020; 11(1):11-24.e4. PMC: 7264033. DOI: 10.1016/j.cels.2020.05.012. View

16.

Lazari L, Ghilardi F, Rosa-Fernandes L, Assis D, Nicolau J, Feijoli Santiago V . Prognostic accuracy of MALDI-TOF mass spectrometric analysis of plasma in COVID-19. Life Sci Alliance. 2021; 4(8). PMC: 8321665. DOI: 10.26508/lsa.202000946. View

17.

Laudanski K, Jihane H, Antalosky B, Ghani D, Phan U, Hernandez R . Unbiased Analysis of Temporal Changes in Immune Serum Markers in Acute COVID-19 Infection With Emphasis on Organ Failure, Anti-Viral Treatment, and Demographic Characteristics. Front Immunol. 2021; 12:650465. PMC: 8226183. DOI: 10.3389/fimmu.2021.650465. View

18.

Memon D, Barrio-Hernandez I, Beltrao P . Individual COVID-19 disease trajectories revealed by plasma proteomics. EMBO Mol Med. 2021; 13(8):e14532. PMC: 8687119. DOI: 10.15252/emmm.202114532. View