Structure Elucidation and Anti-Tumor Activities of Trichothecenes from Endophytic Fungus

Overview

Journal Biomolecules

Publisher MDPI

Specialties Biochemistry
Molecular Biology

Date 2022 Jun 24

PMID 35740903

Authors

Ya-Jing Wang

Nan Ma

Yong-Fu Lu

Si-Yang Dai

Xue Song

Chang Li

Yi Sun

Yue-Hu Pei

Affiliations

Soon will be listed here.

Abstract

The secondary metabolites of , an endophytic fungus with anti-tumor activity isolated from Tsiang, were investigated. Five trichothecenes, including one previously undescribed metabolite, were isolated and identified. Their structures were elucidated by means of extensive spectroscopic methods; the absolute configuration of compound was determined by the ECD method. Surprisingly, 8-n-butyrylneosolaniol () exhibited stronger anti-tumor activity than T-2 toxin against Huh-7 cell line, with an IC value of 265.9 nM. 8-n-butyrylneosolaniol () promoted apoptosis induction in Huh-7 cells. Moreover, cell cycle analysis showed that cell cycle arrest caused by 8-n-butyrylneosolaniol () at the G2/M phase resulted in cell proliferation inhibition and pro-apoptotic activity. Further studies showed a significant decrease in mitochondrial membrane permeabilization and a significant increase in ROS generation, which led to the activation of caspase cascades and subsequent cleavage of PARP fragments. In conclusion, 8-n-butyrylneosolaniol () induced cell apoptosis in Huh-7 cells via the mitochondria-mediated apoptotic signaling pathway, which could be a leading compound for anti-tumor agents.

Citing Articles

Recent Updates on the Secondary Metabolites from Fungi and Their Biological Activities (Covering 2019 to 2024).

Amuzu P, Pan X, Hou X, Sun J, Jakada M, Odigie E J Fungi (Basel). 2024; 10(11).

PMID: 39590697 PMC: 11596042. DOI: 10.3390/jof10110778.

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