The First Optimization Process from Cultivation to Flavonoid-Rich Extract from Lam. Leaves in Brazil

Overview

Journal Foods

Specialty Biotechnology

Date 2022 May 28

PMID 35627022

Authors

Larissa Marina Pereira Silva

Maria Raquel Cavalcanti Inacio

Gualter Guenter Costa da Silva

Jucier Magson de Souza E Silva

Jefferson Romaryo Duarte da Luz

Maria das Gracas Almeida

Edgar Perin Moraes

Debora Esposito

Leandro De Santis Ferreira

Silvana Maria Zucolotto

Affiliations

Soon will be listed here.

Abstract

Flavonoids are significant antioxidant and anti-inflammatory agents and have multiple potential health applications. is globally recognized for its nutritional and pharmacological properties, correlated to the high flavonoid content in its leaves. However, the bioactive compounds found in plants may vary according to the cultivation, origin, season, and extraction process used, making it difficult to extract reliable raw material. Hence, this study aimed to standardize the best cultivation and harvest season in Brazil and the best extraction process conditions to obtain a flavonoid-rich extract from as a final product. Firstly, ultrasound-assisted extraction (UAE) was optimized to reach the highest flavonoid content by three-level factorial planning and response surface methodology (RSM). The optimal cultivation condition was mineral soil fertilizer in the drought season, and the optimized extraction was with 80% ethanol and 13.4 min of extraction time. The flavonoid-rich extract was safe and significantly decreased reactive oxygen species (ROS) and nitric oxide (NO) in LPS-treated RAW 264.7 cells. Lastly, the major flavonoids characterized by HPLC-ESI-QTRAP-MS/MS were compounds derived from apigenin, quercetin, and kaempferol glycosides. The results confirmed that it was possible to standardize the flavonoid-rich extract leading to a standardized and reliable raw material extracted from leaves.

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