Chemical Characterization of Flowers and Leaf Extracts Obtained from and Their Immunomodulatory Effect on LPS-Activated RAW 264.7 Macrophages

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Feb 15

PMID 35164352

Authors

Jefferson Romaryo Duarte da Luz

Eder A Barbosa

Thayse Evellyn Silva do Nascimento

Adriana Augusto de Rezende

Marcela Abbott Galvao Ururahy

Adriana da Silva Brito

Gabriel Araujo-Silva

Jorge A Lopez

Maria das Gracas Almeida

Affiliations

Soon will be listed here.

Abstract

The anti-inflammatory properties of have been evaluated as an alternative drug approach to treating several inflammatory processes. Accordingly, in this study, aqueous and hydroalcoholic extracts of flowers and leaves were analyzed regarding their phytocomposition by ultrafast liquid chromatography coupled to mass spectrometry, and their anti-inflammatory properties were assessed by an in vitro inflammation model, using LPS-stimulated RAW-264.7 macrophages. The phytochemical profile indicated vitexin-2--rhamnoside as an important constituent in both extracts, while methoxyisoflavones, some bulky amino acids (e.g., tryptophan, tyrosine, phenylalanine), pheophorbides, and octadecatrienoic, stearidonic, and ferulic acids were detected in hydroalcoholic extracts. The extracts displayed the ability to modulate the in vitro inflammatory response by altering the secretion of proinflammatory (TNF-α, IL-1β, and IL-6) and anti-inflammatory (IL-10) cytokines and inhibiting the PGE-2 and NO production. Overall, for the first time, putative compounds from flowers and leaves were characterized, which can modulate the inflammatory process. Therefore, the data highlight this plant as an option to obtain extracts for phytotherapic formulations to treat and/or prevent chronic diseases.

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