Chitosan@Puerarin Hydrogel for Accelerated Wound Healing in Diabetic Subjects by MiR-29ab1 Mediated Inflammatory Axis Suppression

Overview

Journal Bioact Mater

Specialty Biomedical Engineering

Date 2022 May 23

PMID 35600974

Authors

Xiaoling Zeng

Baohui Chen

Luping Wang

Yingxiao Sun

Zhao Jin

Xuanyong Liu

Liping Ouyang

Yun Liao

Affiliations

Soon will be listed here.

Abstract

Wound healing is one of the major global health concerns in patients with diabetes. Overactivation of pro-inflammatory M1 macrophages is associated with delayed wound healing in diabetes. miR-29ab1 plays a critical role in diabetes-related macrophage inflammation. Hence, inhibition of inflammation and regulation of miR-29 expression have been implicated as new points for skin wound healing. In this study, the traditional Chinese medicine, puerarin, was introduced to construct an injectable and self-healing chitosan@puerarin (C@P) hydrogel. The C@P hydrogel promoted diabetic wound healing and accelerated angiogenesis, which were related to the inhibition of the miR-29 mediated inflammation response. Compared to healthy subjects, miR-29a and miR-29b1 were ectopically increased in the skin wound of the diabetic model, accompanied by upregulated M1-polarization, and elevated levels of IL-1β and TNF-α. Further evaluations by miR-29ab1 knockout mice exhibited superior wound healing and attenuated inflammation. The present results suggested that miR-29ab1 is essential for diabetic wound healing by regulating the inflammatory response. Suppression of miR-29ab1 by the C@P hydrogel has the potential for improving medical approaches for wound repair.

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