FGFR3 is Expressed by Human Primordial Germ Cells and is Repressed After Meiotic Initiation to Form Primordial Oocytes

Overview

Journal Stem Cell Reports

Publisher Cell Press

Specialty Cell Biology

Date 2022 May 20

PMID 35594860

Authors

Tsotne Chitiashvili

Fei-Man Hsu

Iris Dror

Kathrin Plath

Amander Clark

Affiliations

Soon will be listed here.

Abstract

Human germ cell development is a highly regulated process beginning soon after embryo implantation with the specification of primordial germ cells (PGCs) and ending in adulthood with the differentiation of gametes. Here, we show that fibroblast growth factor receptor 3 (FGFR3) is expressed by human PGCs during the first and second trimester, becoming repressed as PGCs differentiate into primordial oocytes. Using fluorescence-activated cell sorting (FACS) with antibodies that recognize FGFR3 followed by single-cell RNA sequencing, we show that isolating FGFR3-positive cells enriches for human PGCs. Taken together, FGFR3 could be used in future studies as a strategy to identify maturing PGCs in vitro.

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