Defining the Skeletal Myogenic Lineage in Human Pluripotent Stem Cell-Derived Teratomas

Overview

Journal Cells

Publisher MDPI

Specialties Biochemistry
Biophysics
Cell Biology
Molecular Biology

Date 2022 May 14

PMID 35563894

Authors

Matthew P Pappas

Ning Xie

Jacqueline S Penaloza

Sunny S K Chan

Affiliations

Soon will be listed here.

Abstract

Skeletal muscle stem cells are essential to muscle homeostasis and regeneration after injury, and have emerged as a promising cell source for treating skeletal disorders. An attractive approach to obtain these cells utilizes differentiation of pluripotent stem cells (PSCs). We recently reported that teratomas derived from mouse PSCs are a rich source of skeletal muscle stem cells. Here, we showed that teratoma formation is also capable of producing skeletal myogenic progenitors from human PSCs. Using single-cell transcriptomics, we discovered several distinct skeletal myogenic subpopulations that represent progressive developmental stages of the skeletal myogenic lineage and recapitulate human embryonic skeletal myogenesis. We further discovered that ERBB3 and CD82 are effective surface markers for prospective isolation of the skeletal myogenic lineage in human PSC-derived teratomas. Therefore, teratoma formation provides an accessible model for obtaining human skeletal myogenic progenitors from PSCs.

Citing Articles

PSC differentiation as a platform to identify factors for improving the engraftability of cultured muscle stem cells.

Xie N, Robinson K, Sundquist T, Chan S Front Cell Dev Biol. 2024; 12:1362671.

PMID: 38425500 PMC: 10902072. DOI: 10.3389/fcell.2024.1362671.

Efficient Muscle Regeneration by Human PSC-Derived CD82 ERBB3 NGFR Skeletal Myogenic Progenitors.

Xie N, Chu S, Schultz C, Chan S Cells. 2023; 12(3).

PMID: 36766703 PMC: 9913306. DOI: 10.3390/cells12030362.

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