High-Yield Purification, Preservation, and Serial Transplantation of Human Satellite Cells

Overview

Journal Stem Cell Reports

Publisher Cell Press

Specialty Cell Biology

Date 2018 Feb 27

PMID 29478895

Citations 34

Authors

Steven M Garcia

Stanley Tamaki

Solomon Lee

Alvin Wong

Anthony Jose

Joanna Dreux

Gayle Kouklis

Hani Sbitany

Rahul Seth

P Daniel Knott

Chase Heaton

William R Ryan

Esther A Kim

Scott L Hansen

William Y Hoffman

Jason H Pomerantz

Affiliations

Soon will be listed here.

Abstract

Investigation of human muscle regeneration requires robust methods to purify and transplant muscle stem and progenitor cells that collectively constitute the human satellite cell (HuSC) pool. Existing approaches have yet to make HuSCs widely accessible for researchers, and as a result human muscle stem cell research has advanced slowly. Here, we describe a robust and predictable HuSC purification process that is effective for each human skeletal muscle tested and the development of storage protocols and transplantation models in dystrophin-deficient and wild-type recipients. Enzymatic digestion, magnetic column depletion, and 6-marker flow-cytometric purification enable separation of 10 highly enriched HuSCs per gram of muscle. Cryostorage of HuSCs preserves viability, phenotype, and transplantation potential. Development of enhanced and species-specific transplantation protocols enabled serial HuSC xenotransplantation and recovery. These protocols and models provide an accessible system for basic and translational investigation and clinical development of HuSCs.

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