User-friendly Point-of-care Detection of Influenza A (H1N1) Virus Using Light Guide in Three-dimensional Photonic Crystal

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 May 11

PMID 35540156

Authors

Nuree Lee

Cong Wang

Jungyul Park

Affiliations

Soon will be listed here.

Abstract

This paper proposes a user-friendly and highly sensitive detection method for influenza A (H1N1) virus using the cooperation of quantum dot (Qdot)-aptamer beacons and light guide in a three-dimensional (3D) photonic crystal (PC). For easy use, we present a fluorescent probe-based 'OFF-ON' detection protocol. First, a mixture composed of Qdot-aptamer beacons and dark quencher-labeled guard DNA (G-DNA) was prepared. It initially quenched the fluorescent signals to significantly low intensity, , 'OFF' state. Then, the influenza A (H1N1) virus preferentially bound to the aptamer and G-DNA was released, so that the fluorescent signal was restored and biosensor turned to 'ON' state. The restored fluorescence signal changed quantitatively according to the concentration of the target influenza A (H1N1) virus. Owing to the light guide of the 3D nanoporous PC structure, we achieved high sensitivity with ultra-low limit of detection (LOD) of 138 pg mL and high selectivity over other species of influenza A virus and biomolecules. Additionally, with the benefit of enhanced output fluorescent signals, the target virus could be easily detected with a low-cost and portable home-made setup (total cost of only 20 US dollars) and a built-in camera in a smartphone.

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