Point-of-care Diagnostics for Infectious Diseases: From Methods to Devices

Overview

Journal Nano Today

Specialty Biotechnology

Date 2021 Feb 15

PMID 33584847

Citations 135

Authors

Chao Wang

Mei Liu

Zhifei Wang

Song Li

Yan Deng

Nongyue He

Affiliations

Soon will be listed here.

Abstract

The current widespread of COVID-19 all over the world, which is caused by SARS-CoV-2 virus, has again emphasized the importance of development of point-of-care (POC) diagnostics for timely prevention and control of the pandemic. Compared with labor- and time-consuming traditional diagnostic methods, POC diagnostics exhibit several advantages such as faster diagnostic speed, better sensitivity and specificity, lower cost, higher efficiency and ability of on-site detection. To achieve POC diagnostics, developing POC detection methods and correlated POC devices is the key and should be given top priority. The fast development of microfluidics, micro electro-mechanical systems (MEMS) technology, nanotechnology and materials science, have benefited the production of a series of portable, miniaturized, low cost and highly integrated POC devices for POC diagnostics of various infectious diseases. In this review, various POC detection methods for the diagnosis of infectious diseases, including electrochemical biosensors, fluorescence biosensors, surface-enhanced Raman scattering (SERS)-based biosensors, colorimetric biosensors, chemiluminiscence biosensors, surface plasmon resonance (SPR)-based biosensors, and magnetic biosensors, were first summarized. Then, recent progresses in the development of POC devices including lab-on-a-chip (LOC) devices, lab-on-a-disc (LOAD) devices, microfluidic paper-based analytical devices (PADs), lateral flow devices, miniaturized PCR devices, and isothermal nucleic acid amplification (INAA) devices, were systematically discussed. Finally, the challenges and future perspectives for the design and development of POC detection methods and correlated devices were presented. The ultimate goal of this review is to provide new insights and directions for the future development of POC diagnostics for the management of infectious diseases and contribute to the prevention and control of infectious pandemics like COVID-19.

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References

Gutierrez-Sanz O, Andoy N, Filipiak M, Haustein N, Tarasov A . Direct, Label-Free, and Rapid Transistor-Based Immunodetection in Whole Serum. ACS Sens. 2017; 2(9):1278-1286. DOI: 10.1021/acssensors.7b00187. View

Calvert A, Biggerstaff B, Tanner N, Lauterbach M, Lanciotti R . Rapid colorimetric detection of Zika virus from serum and urine specimens by reverse transcription loop-mediated isothermal amplification (RT-LAMP). PLoS One. 2017; 12(9):e0185340. PMC: 5612724. DOI: 10.1371/journal.pone.0185340. View

Kurihara T, Yanagida A, Yokoi H, Koyata A, Matsuya T, Ogawa J . Evaluation of cardiac assays on a benchtop chemiluminescent enzyme immunoassay analyzer, PATHFAST. Anal Biochem. 2008; 375(1):144-6. DOI: 10.1016/j.ab.2007.12.030. View

Zhao C, Liu X . A portable paper-based microfluidic platform for multiplexed electrochemical detection of human immunodeficiency virus and hepatitis C virus antibodies in serum. Biomicrofluidics. 2016; 10(2):024119. PMC: 4833732. DOI: 10.1063/1.4945311. View

Chan J, Yuan S, Kok K, To K, Chu H, Yang J . A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster. Lancet. 2020; 395(10223):514-523. PMC: 7159286. DOI: 10.1016/S0140-6736(20)30154-9. View

Muhlig A, Bocklitz T, Labugger I, Dees S, Henk S, Richter E . LOC-SERS: A Promising Closed System for the Identification of Mycobacteria. Anal Chem. 2016; 88(16):7998-8004. DOI: 10.1021/acs.analchem.6b01152. View

Hu J, Cui X, Gong Y, Xu X, Gao B, Wen T . Portable microfluidic and smartphone-based devices for monitoring of cardiovascular diseases at the point of care. Biotechnol Adv. 2016; 34(3):305-20. DOI: 10.1016/j.biotechadv.2016.02.008. View

Pereira D, Chiu R, Zhang S, Wu B, Kamei D . Single-step, paper-based concentration and detection of a malaria biomarker. Anal Chim Acta. 2015; 882:83-9. DOI: 10.1016/j.aca.2015.04.040. View

Rutledge G, Bohme U, Sanders M, Reid A, Cotton J, Maiga-Ascofare O . Plasmodium malariae and P. ovale genomes provide insights into malaria parasite evolution. Nature. 2017; 542(7639):101-104. PMC: 5326575. DOI: 10.1038/nature21038. View

10.

Tang Y, Li B, Dai J, Dai J, Wang X, Si J . Genotyping of Pseudomonas aeruginosa Type III Secretion System Using Magnetic Enrichment Multiplex Polymerase Chain Reaction and Chemiluminescence. J Biomed Nanotechnol. 2016; 12(4):762-9. DOI: 10.1166/jbn.2016.2222. View

11.

Das S, Peck R, Barney R, Jang I, Kahn M, Zhu M . Performance of an ultra-sensitive Plasmodium falciparum HRP2-based rapid diagnostic test with recombinant HRP2, culture parasites, and archived whole blood samples. Malar J. 2018; 17(1):118. PMC: 5857316. DOI: 10.1186/s12936-018-2268-7. View

12.

Wan L, Gao J, Chen T, Dong C, Li H, Wen Y . LampPort: a handheld digital microfluidic device for loop-mediated isothermal amplification (LAMP). Biomed Microdevices. 2019; 21(1):9. DOI: 10.1007/s10544-018-0354-9. View

13.

Wu Z, McGoogan J . Characteristics of and Important Lessons From the Coronavirus Disease 2019 (COVID-19) Outbreak in China: Summary of a Report of 72 314 Cases From the Chinese Center for Disease Control and Prevention. JAMA. 2020; 323(13):1239-1242. DOI: 10.1001/jama.2020.2648. View

14.

Craw P, Balachandran W . Isothermal nucleic acid amplification technologies for point-of-care diagnostics: a critical review. Lab Chip. 2012; 12(14):2469-86. DOI: 10.1039/c2lc40100b. View

15.

Huang R, He L, Li S, Liu H, Jin L, Chen Z . A simple fluorescence aptasensor for gastric cancer exosome detection based on branched rolling circle amplification. Nanoscale. 2020; 12(4):2445-2451. DOI: 10.1039/c9nr08747h. View

16.

Xi Z, Huang R, Li Z, He N, Wang T, Su E . Selection of HBsAg-Specific DNA Aptamers Based on Carboxylated Magnetic Nanoparticles and Their Application in the Rapid and Simple Detection of Hepatitis B Virus Infection. ACS Appl Mater Interfaces. 2015; 7(21):11215-23. DOI: 10.1021/acsami.5b01180. View

17.

Kim T, Park J, Kim C, Cho Y . Fully integrated lab-on-a-disc for nucleic acid analysis of food-borne pathogens. Anal Chem. 2014; 86(8):3841-8. DOI: 10.1021/ac403971h. View

18.

Kim H, Ren W, Kim J, Yoon J . Fluorescent and colorimetric sensors for detection of lead, cadmium, and mercury ions. Chem Soc Rev. 2011; 41(8):3210-44. DOI: 10.1039/c1cs15245a. View

19.

Chan H, Tan M, Wu H . Point-of-care testing: applications of 3D printing. Lab Chip. 2017; 17(16):2713-2739. DOI: 10.1039/c7lc00397h. View

20.

Shi L, Xu L, Xiao R, Zhou Z, Wang C, Wang S . Rapid, Quantitative, High-Sensitive Detection of O157:H7 by Gold-Shell Silica-Core Nanospheres-Based Surface-Enhanced Raman Scattering Lateral Flow Immunoassay. Front Microbiol. 2020; 11:596005. PMC: 7677456. DOI: 10.3389/fmicb.2020.596005. View