Colloidal Silver-based Lateral Flow Immunoassay for Detection of Profenofos Pesticide Residue in Vegetables

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 May 2

PMID 35497005

Authors

Kuo-Hui Wu

Wen-Chien Huang

Shu-Chen Chang

Rong-Hwa Shyu

Affiliations

Soon will be listed here.

Abstract

A colloidal silver nanoparticle (AgNP)-based lateral flow immunoassay (LFIA) was evaluated in terms of the rapid detection of profenofos (PEO) pesticide residue in vegetables. Colloidal AgNPs, of a diameter of approximately 20 nm, were surface-modified with trisodium citrate dehydrate (TSC) in order to improve their stability and dispersion. An anti-profenofos polyclonal antibody (pAb) was successfully immobilized on the surface of the AgNPs by ionic interaction and characterized using UV-vis, SEM, TEM, FTIR and XPS analyses. Surface modification of Ag-pAb conjugates of varying pH, pAb content and cross-reactivity was employed to design and prepare labels for use in an LFIA to examine whether these factors affect the performance of the assay. The visible detection limit and optical detection limit of the PEO test strip were 0.20 and 0.01 ppm, respectively, in PEO standard solution. This assay showed no cross-reaction with omethoate, methamidophos or pyraclofos. Finally, the PEO test strip was effectively applied for the detection of PEO in liquid vegetables A and B, with optical detection limits of 0.09 and 0.075 ppm, respectively.

Citing Articles

Lateral flow assays: Progress and evolution of recent trends in point-of-care applications.

Kakkar S, Gupta P, Singh Yadav S, Raj D, Singh G, Chauhan S Mater Today Bio. 2024; 28:101188.

PMID: 39221210 PMC: 11364909. DOI: 10.1016/j.mtbio.2024.101188.

Optimized detection of Salmonella typhimurium using aptamer lateral flow assay.

Abedi N, Zeinoddini M, Shoushtari M Biotechnol Lett. 2024; 46(4):583-592.

PMID: 38806936 DOI: 10.1007/s10529-024-03484-1.

Point-of-care detection of Monkeypox virus clades using high-performance upconversion nanoparticle-based lateral flow assay.

Jin B, Ma C, Zhang C, Yin H, Zhao G, Hu J Mikrochim Acta. 2024; 191(4):177.

PMID: 38441684 DOI: 10.1007/s00604-024-06241-3.

An electrochemical immunosensor based on a carboxylated multiwalled carbon nanotube-silver nanoparticle-chitosan functional layer for the detection of fipronil.

Huang W, Hsiung Y, Li C Nanoscale Adv. 2023; 5(23):6548-6559.

PMID: 38024294 PMC: 10662075. DOI: 10.1039/d3na00539a.

Rapid and sensitive detection of E. coli O157:H7 by lateral flow immunoassay and silver enhancement.

Bazsefidpar S, Serrano-Pertierra E, Gutierrez G, Calvo A, Matos M, Blanco-Lopez M Mikrochim Acta. 2023; 190(7):264.

PMID: 37336818 PMC: 10279566. DOI: 10.1007/s00604-023-05834-8.

References

Bai W, Zhu C, Liu J, Yan M, Yang S, Chen A . Gold nanoparticle-based colorimetric aptasensor for rapid detection of six organophosphorous pesticides. Environ Toxicol Chem. 2015; 34(10):2244-9. DOI: 10.1002/etc.3088. View

Luo K, Kim H, Oh M, Kim Y . Paper-based lateral flow strip assay for the detection of foodborne pathogens: principles, applications, technological challenges and opportunities. Crit Rev Food Sci Nutr. 2018; 60(1):157-170. DOI: 10.1080/10408398.2018.1516623. View

Chou C, Huang T, Hsieh P, Ho N, Chen C, Wu K . Quantitative lateral flow immunoassay for rapid detection and monitoring of cerebrospinal fluid leakage following incidental durotomy. Anal Chim Acta. 2022; 1196:339544. DOI: 10.1016/j.aca.2022.339544. View

Yin M, Nie Y, Liu H, Liu L, Tang L, Dong Y . Development of a europium nanoparticles lateral flow immunoassay for NGAL detection in urine and diagnosis of acute kidney injury. BMC Nephrol. 2022; 23(1):30. PMC: 8758895. DOI: 10.1186/s12882-021-02493-w. View

Urusov A, Zherdev A, Dzantiev B . Towards Lateral Flow Quantitative Assays: Detection Approaches. Biosensors (Basel). 2019; 9(3). PMC: 6784366. DOI: 10.3390/bios9030089. View

Monopoli M, Aberg C, Salvati A, Dawson K . Biomolecular coronas provide the biological identity of nanosized materials. Nat Nanotechnol. 2012; 7(12):779-86. DOI: 10.1038/nnano.2012.207. View

Jung Y, Heo Y, Lee J, Deering A, Bae E . Smartphone-based lateral flow imaging system for detection of food-borne bacteria E.coli O157:H7. J Microbiol Methods. 2019; 168:105800. DOI: 10.1016/j.mimet.2019.105800. View

Gebregeorgis A, Bhan C, Wilson O, Raghavan D . Characterization of Silver/Bovine Serum Albumin (Ag/BSA) nanoparticles structure: morphological, compositional, and interaction studies. J Colloid Interface Sci. 2012; 389(1):31-41. DOI: 10.1016/j.jcis.2012.08.041. View

Wang Y, Liu X, Chen C, Chen Y, Li Y, Ye H . Magnetic Nanorobots as Maneuverable Immunoassay Probes for Automated and Efficient Enzyme Linked Immunosorbent Assay. ACS Nano. 2022; 16(1):180-191. DOI: 10.1021/acsnano.1c05267. View

10.

Huang Y, Xu T, Wang W, Wen Y, Li K, Qian L . Lateral flow biosensors based on the use of micro- and nanomaterials: a review on recent developments. Mikrochim Acta. 2019; 187(1):70. DOI: 10.1007/s00604-019-3822-x. View

11.

Bu T, Yao X, Huang L, Dou L, Zhao B, Yang B . Dual recognition strategy and magnetic enrichment based lateral flow assay toward Salmonella enteritidis detection. Talanta. 2019; 206:120204. DOI: 10.1016/j.talanta.2019.120204. View

12.

Cutillas V, Ferrer C, Fernandez-Alba A . Liquid chromatography versus supercritical fluid chromatography coupled to mass spectrometry: a comparative study of performance for multiresidue analysis of pesticides. Anal Bioanal Chem. 2021; 413(23):5849-5857. PMC: 8437864. DOI: 10.1007/s00216-021-03565-4. View

13.

Li Y, Liu L, Song S, Kuang H, Xu C . A Rapid and Semi-Quantitative Gold Nanoparticles Based Strip Sensor for Polymyxin B Sulfate Residues. Nanomaterials (Basel). 2018; 8(3). PMC: 5869635. DOI: 10.3390/nano8030144. View

14.

Hooshmand N, El-Sayed M . Collective multipole oscillations direct the plasmonic coupling at the nanojunction interfaces. Proc Natl Acad Sci U S A. 2019; 116(39):19299-19304. PMC: 6765250. DOI: 10.1073/pnas.1909416116. View

15.

Matea C, Mocan T, Zaharie F, Iancu C, Mocan L . A novel immunoglobulin G monolayer silver bio-nanocomposite. Chem Cent J. 2015; 9(1):55. PMC: 4595522. DOI: 10.1186/s13065-015-0126-z. View

16.

Raysyan A, Schneider R . Development of a Lateral Flow Immunoassay (LFIA) to Screen for the Release of the Endocrine Disruptor Bisphenol A from Polymer Materials and Products. Biosensors (Basel). 2021; 11(7). PMC: 8301804. DOI: 10.3390/bios11070231. View

17.

Liu C, Jia Q, Yang C, Qiao R, Jing L, Wang L . Lateral flow immunochromatographic assay for sensitive pesticide detection by using Fe3O4 nanoparticle aggregates as color reagents. Anal Chem. 2011; 83(17):6778-84. DOI: 10.1021/ac201462d. View

18.

Uznanski P, Zakrzewska J, Favier F, Kazmierski S, Bryszewska E . Synthesis and characterization of silver nanoparticles from (bis)alkylamine silver carboxylate precursors. J Nanopart Res. 2017; 19(3):121. PMC: 5364236. DOI: 10.1007/s11051-017-3827-5. View

19.

Govindaraju S, Ankireddy S, Viswanath B, Kim J, Yun K . Fluorescent Gold Nanoclusters for Selective Detection of Dopamine in Cerebrospinal fluid. Sci Rep. 2017; 7:40298. PMC: 5220289. DOI: 10.1038/srep40298. View

20.

Wang J, Zhang L, Huang Y, Dandapat A, Dai L, Zhang G . Hollow Au-Ag Nanoparticles Labeled Immunochromatography Strip for Highly Sensitive Detection of Clenbuterol. Sci Rep. 2017; 7:41419. PMC: 5278391. DOI: 10.1038/srep41419. View