Handheld Laser Scanning Microscope Catheter for Real-time and Confocal Microscopy Using a High Definition High Frame Rate Lissajous MEMS Mirror

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2022 Apr 13

PMID 35414975

Authors

Jaehun Jeon

Hyunwoo Kim

Hyunwoo Jang

Kyungmin Hwang

Kyuyoung Kim

Young-Gyun Park

Ki-Hun Jeong

Affiliations

Soon will be listed here.

Abstract

A handheld confocal microscope using a rapid MEMS scanning mirror facilitates real-time optical biopsy for simple cancer diagnosis. Here we report a handheld confocal microscope catheter using high definition and high frame rate (HDHF) Lissajous scanning MEMS mirror. The broad resonant frequency region of the fast axis on the MEMS mirror with a low Q-factor facilitates the flexible selection of scanning frequencies. HDHF Lissajous scanning was achieved by selecting the scanning frequencies with high greatest common divisor (GCD) and high total lobe number. The MEMS mirror was fully packaged into a handheld configuration, which was coupled to a home-built confocal imaging system. The confocal microscope catheter allows fluorescence imaging of and mouse tissues with 30 frame rate and 95.4% fill factor at 256 × 256 pixels image, where the lateral resolution is 4.35 μ and the field-of-view (FOV) is 330 × 330 . This compact confocal microscope can provide diverse handheld microscopic applications for real-time, on-demand, and optical biopsy.

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References

Seo Y, Hwang K, Kim H, Jeong K . Scanning MEMS Mirror for High Definition and High Frame Rate Lissajous Patterns. Micromachines (Basel). 2019; 10(1). PMC: 6356757. DOI: 10.3390/mi10010067. View

Shahid W, Qiu Z, Duan X, Li H, Wang T, Oldham K . Modeling and Simulation of a Parametrically Resonant Micromirror With Duty-Cycled Excitation. J Microelectromech Syst. 2014; 23(6):1440-1453. PMC: 4262121. DOI: 10.1109/JMEMS.2014.2315518. View

Kim P, Puorishaag M, Cote D, Lin C, Yun S . In vivo confocal and multiphoton microendoscopy. J Biomed Opt. 2008; 13(1):010501. PMC: 2752311. DOI: 10.1117/1.2839043. View

Schulz-Hildebrandt H, Pfeiffer T, Eixmann T, Lohmann S, Ahrens M, Rehra J . High-speed fiber scanning endoscope for volumetric multi-megahertz optical coherence tomography. Opt Lett. 2018; 43(18):4386-4389. DOI: 10.1364/OL.43.004386. View

Hwang K, Seo Y, Ahn J, Kim P, Jeong K . Frequency selection rule for high definition and high frame rate Lissajous scanning. Sci Rep. 2017; 7(1):14075. PMC: 5658369. DOI: 10.1038/s41598-017-13634-3. View

Liu J, Mandella M, Loewke N, Haeberle H, Ra H, Piyawattanametha W . Micromirror-scanned dual-axis confocal microscope utilizing a gradient-index relay lens for image guidance during brain surgery. J Biomed Opt. 2010; 15(2):026029. PMC: 2869369. DOI: 10.1117/1.3386055. View

Wei L, Yin C, Fujita Y, Sanai N, Liu J . Handheld line-scanned dual-axis confocal microscope with pistoned MEMS actuation for flat-field fluorescence imaging. Opt Lett. 2019; 44(3):671-674. PMC: 7723749. DOI: 10.1364/OL.44.000671. View

Slaoui M, Fiette L . Histopathology procedures: from tissue sampling to histopathological evaluation. Methods Mol Biol. 2010; 691:69-82. DOI: 10.1007/978-1-60761-849-2_4. View

Tanguy Q, Gaiffe O, Passilly N, Cote J, Cabodevila G, Bargiel S . Real-time Lissajous imaging with a low-voltage 2-axis MEMS scanner based on electrothermal actuation. Opt Express. 2020; 28(6):8512-8527. DOI: 10.1364/OE.380690. View

10.

Sullivan S, Muir R, Newman J, Carlsen M, Sreehari S, Doerge C . High frame-rate multichannel beam-scanning microscopy based on Lissajous trajectories. Opt Express. 2014; 22(20):24224-34. PMC: 4247188. DOI: 10.1364/OE.22.024224. View

11.

Jabbour J, Saldua M, Bixler J, Maitland K . Confocal endomicroscopy: instrumentation and medical applications. Ann Biomed Eng. 2011; 40(2):378-97. PMC: 3710661. DOI: 10.1007/s10439-011-0426-y. View

12.

Flores E, Cordova M, Kose K, Phillips W, Rossi A, Nehal K . Intraoperative imaging during Mohs surgery with reflectance confocal microscopy: initial clinical experience. J Biomed Opt. 2015; 20(6):61103. PMC: 4405085. DOI: 10.1117/1.JBO.20.6.061103. View

13.

Hwang K, Seo Y, Kim D, Ahn J, Lee S, Han K . Handheld endomicroscope using a fiber-optic harmonograph enables real-time and in vivo confocal imaging of living cell morphology and capillary perfusion. Microsyst Nanoeng. 2021; 6:72. PMC: 8433427. DOI: 10.1038/s41378-020-00182-6. View

14.

Park H, Song C, Kang M, Jeong Y, Jeong K . Forward imaging OCT endoscopic catheter based on MEMS lens scanning. Opt Lett. 2012; 37(13):2673-5. DOI: 10.1364/OL.37.002673. View

15.

Lee S, Vinegoni C, Sebas M, Weissleder R . Automated motion artifact removal for intravital microscopy, without a priori information. Sci Rep. 2014; 4:4507. PMC: 3968488. DOI: 10.1038/srep04507. View

16.

Arrasmith C, Dickensheets D, Mahadevan-Jansen A . MEMS-based handheld confocal microscope for in-vivo skin imaging. Opt Express. 2010; 18(4):3805-19. PMC: 3378354. DOI: 10.1364/OE.18.003805. View

17.

Duan X, Li H, Qiu Z, Joshi B, Pant A, Smith A . MEMS-based multiphoton endomicroscope for repetitive imaging of mouse colon. Biomed Opt Express. 2015; 6(8):3074-83. PMC: 4541532. DOI: 10.1364/BOE.6.003074. View

18.

Yao C, Li B, Qiu Z . 2D Au-Coated Resonant MEMS Scanner for NIR Fluorescence Intraoperative Confocal Microscope. Micromachines (Basel). 2019; 10(5). PMC: 6562488. DOI: 10.3390/mi10050295. View

19.

Yin C, Glaser A, Leigh S, Chen Y, Wei L, Pillai P . Miniature in vivo MEMS-based line-scanned dual-axis confocal microscope for point-of-care pathology. Biomed Opt Express. 2016; 7(2):251-63. PMC: 4771446. DOI: 10.1364/BOE.7.000251. View

20.

Yoneya S, Saito T, Komatsu Y, Koyama I, Takahashi K . Binding properties of indocyanine green in human blood. Invest Ophthalmol Vis Sci. 1998; 39(7):1286-90. View