Micromirror-scanned Dual-axis Confocal Microscope Utilizing a Gradient-index Relay Lens for Image Guidance During Brain Surgery

Overview

Journal J Biomed Opt

Specialties Biomedical Engineering
Ophthalmology

Date 2010 May 13

PMID 20459274

Citations 32

Authors

Jonathan T C Liu

Michael J Mandella

Nathan O Loewke

Henry Haeberle

Hyejun Ra

Wibool Piyawattanametha

Olav Solgaard

Gordon S Kino

Christopher H Contag

Affiliations

Soon will be listed here.

Abstract

A fluorescence confocal microscope incorporating a 1.8-mm-diam gradient-index relay lens is developed for in vivo histological guidance during resection of brain tumors. The microscope utilizes a dual-axis confocal architecture to efficiently reject out-of-focus light for high-contrast optical sectioning. A biaxial microelectromechanical system (MEMS) scanning mirror is actuated at resonance along each axis to achieve a large field of view with low-voltage waveforms. The unstable Lissajous scan, which results from actuating the orthogonal axes of the MEMS mirror at highly disparate resonance frequencies, is optimized to fully sample 500x500 pixels at two frames per second. Optically sectioned fluorescence images of brain tissues are obtained in living mice to demonstrate the utility of this microscope for image-guided resections.

Citing Articles

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Correction of non-uniform angular velocity and sub-pixel jitter in optical scanning.

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Wang J, Zhang G, You Z Microsyst Nanoeng. 2021; 6:101.

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