Adaptive Optics Fluorescence Lifetime Imaging Ophthalmoscopy of Human Retinal Pigment Epithelium

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2022 Apr 13

PMID 35414970

Authors

Janet A H Tang

Charles E Granger

Karteek Kunala

Keith Parkins

Khang T Huynh

Kristen Bowles-Johnson

Qiang Yang

Jennifer J Hunter

Affiliations

Soon will be listed here.

Abstract

The intrinsic fluorescence properties of lipofuscin - naturally occurring granules that accumulate in the retinal pigment epithelium - are a potential biomarker for the health of the eye. A new modality is described here which combines adaptive optics technology with fluorescence lifetime detection, allowing for the investigation of functional and compositional differences within the eye and between subjects. This new adaptive optics fluorescence lifetime imaging ophthalmoscope was demonstrated in 6 subjects. Repeated measurements between visits had a minimum intraclass correlation coefficient of 0.59 Although the light levels were well below maximum permissible exposures, the safety of the imaging paradigm was tested using clinical measures; no concerns were raised. This new technology allows for adaptive optics fluorescence lifetime imaging of the human RPE mosaic.

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