Comparison of a Fluorescent Monoclonal Antibody Assay and a Tissue Culture Assay for Routine Detection of Infections Caused by Chlamydia Trachomatis

Overview

Journal Eur J Clin Microbiol

Specialty Microbiology

Date 1986 Oct 1

PMID 3536501

Citations 9

Authors

J H Larsen

H C Wulf

A Friis-Moller

Affiliations

Soon will be listed here.

Abstract

The applicability of a commercial direct immunofluorescent monoclonal antibody assay for detection of Chlamydia trachomatis elementary bodies was studied on endocervical smears from 506 women attending a venereal disease clinic. The aim of this prospective examination was to simulate a daily routine. The results were compared to those of a well-functioning tissue culture assay. The overall positivity was 22.7%. Based on a positivity criterion of greater than or equal to 1 elementary body in the fluorescent antibody assay, the two assays agreed in 84.8% of the cases. In 50 specimens the antibody assay was positive and the culture assay negative, whereas in 23 the culture assay was positive and the antibody assay negative. The positive predictive value was 63.8%. Most of the discrepancies were found in specimens containing few elementary bodies or inclusions. Based on a criterion of greater than or equal to 10 elementary bodies, the positive predictive value was 70.9%, but the sensitivity fell to 67.5%.

Citing Articles

Just the berries. Diagnosing Chlamydia trachomatis.

Hickey J Can Fam Physician. 2002; 47:2229-30.

PMID: 11768920 PMC: 2018469.

Evaluation of the Biostar Chlamydia OIA assay with specimens from women attending a sexually transmitted disease clinic.

Pate M, Dixon P, Hardy K, Crosby M, Hook 3rd E J Clin Microbiol. 1998; 36(8):2183-6.

PMID: 9665987 PMC: 105001. DOI: 10.1128/JCM.36.8.2183-2186.1998.

Diagnosis by AMPLICOR PCR of Chlamydia trachomatis infection in urine samples from women and men attending sexually transmitted disease clinics.

Quinn T, Welsh L, Lentz A, Crotchfelt K, Zenilman J, Newhall J J Clin Microbiol. 1996; 34(6):1401-6.

PMID: 8735088 PMC: 229032. DOI: 10.1128/jcm.34.6.1401-1406.1996.

Periodic health examination, 1996 update: 2. Screening for chlamydial infections. Canadian Task Force on the Periodic Health Examination.

Davies H, Wang E CMAJ. 1996; 154(11):1631-44.

PMID: 8646651 PMC: 1487928.

Assessment of enzyme immunoassay and immunofluorescence tests for detection of Chlamydia trachomatis.

Hipp S, Han Y, Murphy D J Clin Microbiol. 1987; 25(10):1938-43.

PMID: 3312290 PMC: 269372. DOI: 10.1128/jcm.25.10.1938-1943.1987.

References

Munday P, Johnson A, Thomas B, Taylor-Robinson D . A comparison of the sensitivity of immunofluorescence and Giemsa for staining Chlamydia trachomatis inclusions in cycloheximide-treated McCoy cells. J Clin Pathol. 1980; 33(2):177-9. PMC: 1146016. DOI: 10.1136/jcp.33.2.177. View

Hallsworth P, McDonald P . Detection of Chlamydia trachomatis with fluorescent monoclonal antibody. Pathology. 1985; 17(3):500-2. DOI: 10.3109/00313028509105509. View

UYEDA C, Welborn P, Shunk K, Tsaouse B . Rapid diagnosis of chlamydial infections with the MicroTrak direct test. J Clin Microbiol. 1984; 20(5):948-50. PMC: 271481. DOI: 10.1128/jcm.20.5.948-950.1984. View

Harper I, Shearman M, Dalgety F, Cole D . Fluorescein-conjugated monoclonal antibodies to detect Chlamydia trachomatis in smears. Lancet. 1985; 2(8453):509. DOI: 10.1016/s0140-6736(85)90450-7. View

Stamm W, Harrison H, ALEXANDER E, Cles L, Spence M, Quinn T . Diagnosis of Chlamydia trachomatis infections by direct immunofluorescence staining of genital secretions. A multicenter trial. Ann Intern Med. 1984; 101(5):638-41. DOI: 10.7326/0003-4819-101-5-638. View

Mallinson H, TURNER G, Carey P, Khan M . Rapid detection of Chlamydia trachomatis with monoclonal antibodies. Lancet. 1984; 1(8387):1180-1. DOI: 10.1016/s0140-6736(84)91423-5. View

Dutilh B, Bebear C . [Chlamydia trachomatis: detection using a direct test and culture in 100 genital specimens]. Ann Biol Clin (Paris). 1985; 43(3):275-8. View

Wingerson L . Two new tests for chlamydia get quick results without culture. JAMA. 1983; 250(17):2257-9. View

Zapata M, Chernesky M, Mahony J . Indirect immunofluorescence staining of Chlamydia trachomatis inclusions in microculture plates with monoclonal antibodies. J Clin Microbiol. 1984; 19(6):937-9. PMC: 271220. DOI: 10.1128/jcm.19.6.937-939.1984. View

10.

Williams T, MANIAR A, Brunham R, Hammond G . Identification of Chlamydia trachomatis by direct immunofluorescence applied in specimens originating in remote areas. J Clin Microbiol. 1985; 22(6):1053-4. PMC: 271878. DOI: 10.1128/jcm.22.6.1053-1054.1985. View

11.

Krech T, Hofmann N, Miller S . Interference of Staphylococcus aureus in the detection of Chlamydia trachomatis by monoclonal antibodies. Lancet. 1985; 1(8438):1161-2. DOI: 10.1016/s0140-6736(85)92467-5. View

12.

Stamm W, Tam M, Koester M, Cles L . Detection of Chlamydia trachomatis inclusions in Mccoy cell cultures with fluorescein-conjugated monoclonal antibodies. J Clin Microbiol. 1983; 17(4):666-8. PMC: 272712. DOI: 10.1128/jcm.17.4.666-668.1983. View

13.

Kiviat N, Peterson M, Tam M, Stamm W, Holmes K . Cytologic manifestations of cervical and vaginal infections. II. Confirmation of Chlamydia trachomatis infection by direct immunofluorescence using monoclonal antibodies. JAMA. 1985; 253(7):997-1000. DOI: 10.1001/jama.253.7.997. View

14.

Stephens R, Kuo C, Tam M . Sensitivity of immunofluorescence with monoclonal antibodies for detection of Chlamydia trachomatis inclusions in cell culture. J Clin Microbiol. 1982; 16(1):4-7. PMC: 272285. DOI: 10.1128/jcm.16.1.4-7.1982. View

15.

Alexander I, Paul I, Caul E . Evaluation of a genus reactive monoclonal antibody in rapid identification of Chlamydia trachomatis by direct immunofluorescence. Genitourin Med. 1985; 61(4):252-4. PMC: 1011823. DOI: 10.1136/sti.61.4.252. View

16.

Volkert M, Matthiesen M . Ornithosis-related antigens from B. anitratum in the serological diagnosis of ornithosis. I. Acta Pathol Microbiol Scand. 1958; 44(3):278-86. DOI: 10.1111/j.1699-0463.1958.tb01076.x. View

17.

Thomas B, Evans R, Hutchinson G, Taylor-Robinson D . Early detection of chlamydial inclusions combining the use of cycloheximide-treated McCoy cells and immunofluorescence staining. J Clin Microbiol. 1977; 6(3):285-92. PMC: 274754. DOI: 10.1128/jcm.6.3.285-292.1977. View

18.

. Fluorescein-conjugated monoclonal antibodies to detect Chlamydia trachomatis in smears. Lancet. 1985; 2(8448):222-3. View

19.

THORNLEY M, Zamze S, Byrne M, Lusher M, Evans R . Properties of monoclonal antibodies to the genus-specific antigen of Chlamydia and their use for antigen detection by reverse passive haemagglutination. J Gen Microbiol. 1985; 131(1):7-15. DOI: 10.1099/00221287-131-1-7. View

20.

Berron S, Vazquez J, Fenoll A . Rapid detection of Chlamydia trachomatis. Lancet. 1984; 2(8394):109-10. DOI: 10.1016/s0140-6736(84)90285-x. View