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Indirect Immunofluorescence Staining of Chlamydia Trachomatis Inclusions in Microculture Plates with Monoclonal Antibodies

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1984 Jun 1
PMID 6206089
Citations 11
Authors
M Zapata
M Chernesky
J Mahony
Affiliations
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Abstract

Indirect immunofluorescence (IF) staining, using a monoclonal antibody, detected two- to fourfold more inclusions than did iodine staining. Of 274 clinical specimens, 53 (19.3%) were positive by IF on passage 1 as compared with 33 (12%) by iodine staining (P less than 0.005). IF-stained inclusions in McCoy cells in the bottom of microculture wells were readily viewed with a long-focal-length objective at a magnification of 250 X.

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