A Single-cell Atlas of Human and Mouse White Adipose Tissue

Overview

Journal Nature

Specialty Science

Date 2022 Mar 17

PMID 35296864

Authors

Margo P Emont

Christopher Jacobs

Adam L Essene

Deepti Pant

Danielle Tenen

Georgia Colleluori

Angelica Di Vincenzo

Anja M Jorgensen

Hesam Dashti

Adam Stefek

Elizabeth McGonagle

Sophie Strobel

Samantha Laber

Saaket Agrawal

Gregory P Westcott

Amrita Kar

Molly L Veregge

Anton Gulko

Harini Srinivasan

Zachary Kramer

Eleanna De Filippis

Erin Merkel

Jennifer Ducie

Christopher G Boyd

William Gourash

Anita Courcoulas

Samuel J Lin

Bernard T Lee

Donald Morris

Adam Tobias

Amit V Khera

Melina Claussnitzer

Tune H Pers

Antonio Giordano

Orr Ashenberg

Aviv Regev

Linus T Tsai

Evan D Rosen

Affiliations

Soon will be listed here.

Abstract

White adipose tissue, once regarded as morphologically and functionally bland, is now recognized to be dynamic, plastic and heterogenous, and is involved in a wide array of biological processes including energy homeostasis, glucose and lipid handling, blood pressure control and host defence. High-fat feeding and other metabolic stressors cause marked changes in adipose morphology, physiology and cellular composition, and alterations in adiposity are associated with insulin resistance, dyslipidemia and type 2 diabetes. Here we provide detailed cellular atlases of human and mouse subcutaneous and visceral white fat at single-cell resolution across a range of body weight. We identify subpopulations of adipocytes, adipose stem and progenitor cells, vascular and immune cells and demonstrate commonalities and differences across species and dietary conditions. We link specific cell types to increased risk of metabolic disease and provide an initial blueprint for a comprehensive set of interactions between individual cell types in the adipose niche in leanness and obesity. These data comprise an extensive resource for the exploration of genes, traits and cell types in the function of white adipose tissue across species, depots and nutritional conditions.

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