Keratin Filaments Mediate the Expansion of Extra-embryonic Membranes in the Post-gastrulation Mouse Embryo

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2022 Mar 10

PMID 35266581

Authors

Wallis Nahaboo

Sema Elif Eski

Evangeline Despin-Guitard

Marjorie Vermeersch

Marie Versaevel

Bechara Saykali

Daniel Monteyne

Sylvain Gabriele

Thomas M Magin

Nicole Schwarz

Rudolf E Leube

An Zwijsen

David Perez-Morga

Sumeet Pal Singh

Isabelle Migeotte

Affiliations

Soon will be listed here.

Abstract

Mesoderm arises at gastrulation and contributes to both the mouse embryo proper and its extra-embryonic membranes. Two-photon live imaging of embryos bearing a keratin reporter allowed recording filament nucleation and elongation in the extra-embryonic region. Upon separation of amniotic and exocoelomic cavities, keratin 8 formed apical cables co-aligned across multiple cells in the amnion, allantois, and blood islands. An influence of substrate rigidity and composition on cell behavior and keratin content was observed in mesoderm explants. Embryos lacking all keratin filaments displayed a deflated extra-embryonic cavity, a narrow thick amnion, and a short allantois. Single-cell RNA sequencing of sorted mesoderm cells and micro-dissected amnion, chorion, and allantois, provided an atlas of transcriptomes with germ layer and regional information. It defined the cytoskeleton and adhesion expression profile of mesoderm-derived keratin 8-enriched cells lining the exocoelomic cavity. Those findings indicate a novel role for keratin filaments in the expansion of extra-embryonic structures and suggest mechanisms of mesoderm adaptation to the environment.

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Keratin filaments mediate the expansion of extra-embryonic membranes in the post-gastrulation mouse embryo.

Nahaboo W, Eski S, Despin-Guitard E, Vermeersch M, Versaevel M, Saykali B EMBO J. 2022; 41(7):e108747.

PMID: 35266581 PMC: 8982622. DOI: 10.15252/embj.2021108747.

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