Vaccination Accelerates Liver-Intrinsic Expression of Megakaryocyte-Related Genes in Response to Blood-Stage Malaria

Overview

Journal Vaccines (Basel)

Date 2022 Feb 26

PMID 35214745

Authors

Frank Wunderlich

Denis Delic

Daniela Gerovska

Marcos J Arauzo-Bravo

Affiliations

Soon will be listed here.

Abstract

Erythropoiesis and megakaryo-/thrombopoiesis occur in the bone marrow proceeding from common, even bipotent, progenitor cells. Recently, we have shown that protective vaccination accelerates extramedullary hepatic erythroblastosis in response to blood-stage malaria of Here, we investigated whether protective vaccination also accelerates extramedullary hepatic megakaryo-/thrombopoiesis. Female Balb/c mice were twice vaccinated with a non-infectious vaccine before infecting with 10 -parasitized erythrocytes. Using gene expression microarrays and quantitative real-time PCR, transcripts of genes known to be expressed in the bone marrow by cells of the megakaryo-/thrombocytic lineage were compared in livers of vaccination-protected and unprotected mice on days 0, 1, 4, 8, and 11 Livers of vaccination-protected mice responded with expression of megakaryo-/thrombocytic genes faster to than those of unvaccinated mice, evidenced at early patency on day 4 , when livers exhibited significantly higher levels of malaria-induced transcripts of the genes and (-values < 0.0001), (-value < 0.001), and , , , , , , , , , and (-values < 0.01). Together with additionally analyzed genes known to be related to megakaryopoiesis, our data suggest that protective vaccination accelerates liver-intrinsic megakaryo-/thrombopoiesis in response to blood-stage malaria that presumably contributes to vaccination-induced survival of otherwise lethal blood-stage malaria.

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