LINC00467 Facilitates the Proliferation, Migration and Invasion of Glioma Via Promoting the Expression of Inositol Hexakisphosphate Kinase 2 by Binding to MiR-339-3p

Overview

Journal Bioengineered

Date 2022 Feb 14

PMID 35156508

Authors

Yin Zhang

Yaxuan Zhang

Sen Wang

Boqiang Cao

Daling Hu

Junli Jia

Yuhang Wang

Luyao Chen

Jiaming Li

Hongyi Liu

Huamin Tang

Affiliations

Soon will be listed here.

Abstract

Our previous studies indicate that long noncoding RNA (lncRNA) LINC00467 can act as an oncogene to participate in the malignant progression of glioma, but the underlying molecular mechanism remains to be studied further. This study aimed to explore the biological role of the LINC00467/miR-339-3p/ inositol hexakisphosphate kinase 2 (IP6K2) regulatory axis in glioma. The Cancer Genome Atlas (TCGA), Oncomine databases and reverse transcription‑quantitative PCR (RT‑qPCR) were used to analyze IP6K2 expression in glioma. RT-PCR, EdU and transwell assays were conducted to observe the effect of IP6K2 on glioma cell proliferation, migration and invasion. Using bioinformatics analysis, RT-PCR, and dual luciferase reporter gene assay, the potential role of the LINC00467/miR-339-3p/IP6K2 regulatory axis in glioma was verified. The results showed that IP6K2 was up-regulated in glioma tissues and cell lines. Moreover, the expression level of IP6K2 was correlated with the clinical features of glioma patients. and experiments indicated that IP6K2 overexpression could promote the proliferation, migration, and invasion of glioma cells. Further bioinformatics analysis and assays revealed that LINC00467 could promote IP6K2 expression by binding to miR-339-3p and promote the malignant progression of glioma. Overall, LINC00467 could upregulate IP6K2 by binding to miR-339-3p and promote the proliferation, migration, and invasion of glioma cells. The LINC00467/miR-339-3p/IP6K2 regulatory axis might be a potential therapeutic target for glioma.

Citing Articles

Hsp70 and Calcitonin Receptor Protein in Extracellular Vesicles from Glioblastoma Multiforme: Biomarkers with Putative Roles in Carcinogenesis and Potential for Differentiating Tumor Types.

Alberti G, Sanchez-Lopez C, Marcilla A, Barone R, Caruso Bavisotto C, Graziano F Int J Mol Sci. 2024; 25(6).

PMID: 38542389 PMC: 10969952. DOI: 10.3390/ijms25063415.

LINC00467 mediates the 5-fluorouracil resistance in breast cancer cells.

Li L, Zhang Y, Zhan Y, Zhong Y, Li X In Vitro Cell Dev Biol Anim. 2023; 60(1):80-88.

PMID: 38127229 DOI: 10.1007/s11626-023-00832-9.

Functions, Mechanisms, and therapeutic applications of the inositol pyrophosphates 5PP-InsP and InsP in mammalian cells.

Qi J, Shi L, Zhu L, Chen Y, Zhu H, Cheng W J Cardiovasc Transl Res. 2023; 17(1):197-215.

PMID: 37615888 DOI: 10.1007/s12265-023-10427-0.

KCNQ1OT1 promotes retinoblastoma progression by targeting miR-339-3p that suppresses KIF23.

Tang W, Zhang L, Li J, Guan Y Int Ophthalmol. 2023; 43(7):2419-2432.

PMID: 37198502 DOI: 10.1007/s10792-023-02641-1.

Wu S, Ballah A, Che W, Wang X Front Genet. 2022; 13:961278.

PMID: 36468039 PMC: 9708876. DOI: 10.3389/fgene.2022.961278.

References

Cai H, Yu Y, Ni X, Li C, Hu Y, Wang J . LncRNA LINC00998 inhibits the malignant glioma phenotype via the CBX3-mediated c-Met/Akt/mTOR axis. Cell Death Dis. 2020; 11(12):1032. PMC: 7710718. DOI: 10.1038/s41419-020-03247-6. View

Gyoba J, Shan S, Roa W, Bedard E . Diagnosing Lung Cancers through Examination of Micro-RNA Biomarkers in Blood, Plasma, Serum and Sputum: A Review and Summary of Current Literature. Int J Mol Sci. 2016; 17(4):494. PMC: 4848950. DOI: 10.3390/ijms17040494. View

Mu M, Niu W, Zhang X, Hu S, Niu C . LncRNA BCYRN1 inhibits glioma tumorigenesis by competitively binding with miR-619-5p to regulate CUEDC2 expression and the PTEN/AKT/p21 pathway. Oncogene. 2020; 39(45):6879-6892. PMC: 7644463. DOI: 10.1038/s41388-020-01466-x. View

Ballantyne M, McDonald R, Baker A . lncRNA/MicroRNA interactions in the vasculature. Clin Pharmacol Ther. 2016; 99(5):494-501. PMC: 4881297. DOI: 10.1002/cpt.355. View

Zhang X, Li F, Zhou Y, Mao F, Lin Y, Shen S . Long noncoding RNA AFAP1-AS1 promotes tumor progression and invasion by regulating the miR-2110/Sp1 axis in triple-negative breast cancer. Cell Death Dis. 2021; 12(7):627. PMC: 8213778. DOI: 10.1038/s41419-021-03917-z. View

Weber C, Luo C, Hotz-Wagenblatt A, Gardyan A, Kordass T, Holland-Letz T . miR-339-3p Is a Tumor Suppressor in Melanoma. Cancer Res. 2016; 76(12):3562-71. DOI: 10.1158/0008-5472.CAN-15-2932. View

DeSouza P, Qu X, Chen H, Patel B, Maher C, Kim A . Long, Noncoding RNA Dysregulation in Glioblastoma. Cancers (Basel). 2021; 13(7). PMC: 8037018. DOI: 10.3390/cancers13071604. View

Salmena L, Poliseno L, Tay Y, Kats L, Pandolfi P . A ceRNA hypothesis: the Rosetta Stone of a hidden RNA language?. Cell. 2011; 146(3):353-8. PMC: 3235919. DOI: 10.1016/j.cell.2011.07.014. View

Deng S, Zhu S, Qiao Y, Liu Y, Chen W, Zhao G . Recent advances in the role of toll-like receptors and TLR agonists in immunotherapy for human glioma. Protein Cell. 2014; 5(12):899-911. PMC: 4259890. DOI: 10.1007/s13238-014-0112-6. View

10.

Zhu S, Tang X, Gao X, Zhang J, Cui Y, Li D . hsa_circ_0013401 Accelerates the Growth and Metastasis and Prevents Apoptosis and Autophagy of Neuroblastoma Cells by Sponging miR-195 to Release PAK2. Oxid Med Cell Longev. 2021; 2021:9936154. PMC: 8629642. DOI: 10.1155/2021/9936154. View

11.

Zhang Y, Jiang X, Wu Z, Hu D, Jia J, Guo J . Long Noncoding RNA LINC00467 Promotes Glioma Progression through Inhibiting P53 Expression via Binding to DNMT1. J Cancer. 2020; 11(10):2935-2944. PMC: 7086258. DOI: 10.7150/jca.41942. View

12.

Yang Z, Ming Y, Wang T, Zhi T, Zhou Y, Tian M . lncRNA SNHG11 promotes the development of colorectal cancer by mediating miR-339-3p/SHOX2. Minerva Med. 2020; 113(5):898-899. DOI: 10.23736/S0026-4806.20.06619-7. View

13.

Jandura A, Krause H . The New RNA World: Growing Evidence for Long Noncoding RNA Functionality. Trends Genet. 2017; 33(10):665-676. DOI: 10.1016/j.tig.2017.08.002. View

14.

Tang Z, Li C, Kang B, Gao G, Li C, Zhang Z . GEPIA: a web server for cancer and normal gene expression profiling and interactive analyses. Nucleic Acids Res. 2017; 45(W1):W98-W102. PMC: 5570223. DOI: 10.1093/nar/gkx247. View

15.

Cheng J, Meng J, Zhu L, Peng Y . Exosomal noncoding RNAs in Glioma: biological functions and potential clinical applications. Mol Cancer. 2020; 19(1):66. PMC: 7098115. DOI: 10.1186/s12943-020-01189-3. View

16.

Zhao C, Yan Z, Wen J, Fu D, Xu P, Wang L . CircEAF2 counteracts Epstein-Barr virus-positive diffuse large B-cell lymphoma progression via miR-BART19-3p/APC/β-catenin axis. Mol Cancer. 2021; 20(1):153. PMC: 8638185. DOI: 10.1186/s12943-021-01458-9. View

17.

Zhang X, Zhang Z . Oncohistone Mutations in Diffuse Intrinsic Pontine Glioma. Trends Cancer. 2019; 5(12):799-808. PMC: 6986369. DOI: 10.1016/j.trecan.2019.10.009. View

18.

Sallam T, Sandhu J, Tontonoz P . Long Noncoding RNA Discovery in Cardiovascular Disease: Decoding Form to Function. Circ Res. 2018; 122(1):155-166. PMC: 5902384. DOI: 10.1161/CIRCRESAHA.117.311802. View

19.

Zhang Y, Liu Q, Liao Q . Long noncoding RNA: a dazzling dancer in tumor immune microenvironment. J Exp Clin Cancer Res. 2020; 39(1):231. PMC: 7641842. DOI: 10.1186/s13046-020-01727-3. View

20.

Li J, Liu S, Zhou H, Qu L, Yang J . starBase v2.0: decoding miRNA-ceRNA, miRNA-ncRNA and protein-RNA interaction networks from large-scale CLIP-Seq data. Nucleic Acids Res. 2013; 42(Database issue):D92-7. PMC: 3964941. DOI: 10.1093/nar/gkt1248. View