Towards an Ideal Hybridization-Based Strategy to Discover Protein Interactomes of Selected RNA Molecules

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Jan 21

PMID 35055128

Authors

Michele Spiniello

Mark Scalf

Amelia Casamassimi

Ciro Abbondanza

Lloyd M Smith

Affiliations

Soon will be listed here.

Abstract

RNA-binding proteins are crucial to the function of coding and non-coding RNAs. The disruption of RNA-protein interactions is involved in many different pathological states. Several computational and experimental strategies have been developed to identify protein binders of selected RNA molecules. Amongst these, '' hybridization methods represent the gold standard in the field because they are designed to reveal the proteins bound to specific RNAs in a cellular context. Here, we compare the technical features of different ' hybridization approaches with a focus on their advantages, limitations, and current and potential future applications.

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