Human Y5 RNA Specializes a Ro Ribonucleoprotein for 5S Ribosomal RNA Quality Control

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 2007 Dec 7

PMID 18056422

Citations 48

Authors

J Robert Hogg

Kathleen Collins

Affiliations

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Abstract

Humans express four distinct non-protein-coding Y RNAs (ncRNAs). To investigate Y RNA functional diversification, we exploited an RNA-based affinity purification method to isolate ribonucleoproteins (RNPs) assembled on individual human Y RNAs. Silver staining and mass spectrometry revealed that the Ro and La proteins assemble with all Y RNAs, while additional proteins associate with specific Y RNAs. Unexpectedly, Y5 RNA uniquely copurified ribosomal protein L5 and its binding partner 5S RNA. These findings reveal a contribution of Y5 to 5S surveillance and suggest that interactions between Ro-Y5 and L5-5S RNPs establish 5S RNA as a target of quality control.

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