Effect of Nutritional Factors and Copper on the Regulation of Laccase Enzyme Production in

Overview

Journal J Fungi (Basel)

Date 2022 Jan 20

PMID 35049947

Authors

Dinary Duran-Sequeda

Daniela Suspes

Estibenson Maestre

Manuel Alfaro

Gumer Perez

Lucia Ramirez

Antonio G Pisabarro

Rocio Sierra

Affiliations

Soon will be listed here.

Abstract

This research aimed to establish the relationship between carbon-nitrogen nutritional factors and copper sulfate on laccase activity (LA) by  . Culture media composition was tested to choose the nitrogen source. Yeast extract (YE) was selected as a better nitrogen source than ammonium sulfate. Then, the effect of glucose and YE concentrations on biomass production and LA as response variables was evaluated using central composite experimental designs with and without copper. The results showed that the best culture medium composition was glucose 45 gL and YE 15 gL, simultaneously optimizing these two response variables. The fungal transcriptome was obtained in this medium with or without copper, and the differentially expressed genes were found. The main upregulated transcripts included three laccase genes (, , and ) regulated by copper, whereas the principal downregulated transcripts included a copper transporter () and a regulator of nitrogen metabolism (). These results suggest that Ctr1, which facilitates the entry of copper into the cell, is regulated by nutrient-sufficiency conditions. Once inside, copper induces transcription of laccase genes. This finding could explain why a 10-20-fold increase in LA occurs with copper compared to cultures without copper when using the optimal concentration of YE as nitrogen sources.

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References

Giardina P, Faraco V, Pezzella C, Piscitelli A, Vanhulle S, Sannia G . Laccases: a never-ending story. Cell Mol Life Sci. 2009; 67(3):369-85. PMC: 11115910. DOI: 10.1007/s00018-009-0169-1. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Palmieri G, Giardina P, Bianco C, Fontanella B, Sannia G . Copper induction of laccase isoenzymes in the ligninolytic fungus Pleurotus ostreatus. Appl Environ Microbiol. 2000; 66(3):920-4. PMC: 91923. DOI: 10.1128/AEM.66.3.920-924.2000. View

Jiao X, Li G, Wang Y, Nie F, Cheng X, Abdullah M . Systematic Analysis of the Pleurotus ostreatus Laccase Gene (PoLac) Family and Functional Characterization of PoLac2 Involved in the Degradation of Cotton-Straw Lignin. Molecules. 2018; 23(4). PMC: 6017272. DOI: 10.3390/molecules23040880. View

Lettera V, Del Vecchio C, Piscitelli A, Sannia G . Low impact strategies to improve ligninolytic enzyme production in filamentous fungi: the case of laccase in Pleurotus ostreatus. C R Biol. 2011; 334(11):781-8. DOI: 10.1016/j.crvi.2011.06.001. View

Grigoriev I, Nikitin R, Haridas S, Kuo A, Ohm R, Otillar R . MycoCosm portal: gearing up for 1000 fungal genomes. Nucleic Acids Res. 2013; 42(Database issue):D699-704. PMC: 3965089. DOI: 10.1093/nar/gkt1183. View

Grandes-Blanco A, Diaz-Godinez G, Tellez-Tellez M, Delgado-Macuil R, Rojas-Lopez M, Bibbins-Martinez M . Ligninolytic activity patterns of Pleurotus ostreatus obtained by submerged fermentation in presence of 2,6-dimethoxyphenol and remazol brilliant blue R dye. Prep Biochem Biotechnol. 2013; 43(5):468-80. DOI: 10.1080/10826068.2012.746233. View

Faraco V, Ercole C, Festa G, Giardina P, Piscitelli A, Sannia G . Heterologous expression of heterodimeric laccase from Pleurotus ostreatus in Kluyveromyces lactis. Appl Microbiol Biotechnol. 2007; 77(6):1329-35. DOI: 10.1007/s00253-007-1265-5. View

Larraya L, Perez G, Penas M, Baars J, Mikosch T, Pisabarro A . Molecular karyotype of the white rot fungus Pleurotus ostreatus. Appl Environ Microbiol. 1999; 65(8):3413-7. PMC: 91513. DOI: 10.1128/AEM.65.8.3413-3417.1999. View

10.

Fernandez-Fueyo E, Ruiz-Duenas F, Lopez-Lucendo M, Perez-Boada M, Rencoret J, Gutierrez A . A secretomic view of woody and nonwoody lignocellulose degradation by Pleurotus ostreatus. Biotechnol Biofuels. 2016; 9:49. PMC: 4772462. DOI: 10.1186/s13068-016-0462-9. View

11.

Tudzynski B . Nitrogen regulation of fungal secondary metabolism in fungi. Front Microbiol. 2014; 5:656. PMC: 4246892. DOI: 10.3389/fmicb.2014.00656. View

12.

Morgulis A, Coulouris G, Raytselis Y, Madden T, Agarwala R, Schaffer A . Database indexing for production MegaBLAST searches. Bioinformatics. 2008; 24(16):1757-64. PMC: 2696921. DOI: 10.1093/bioinformatics/btn322. View

13.

Aza P, de Salas F, Molpeceres G, Rodriguez-Escribano D, de la Fuente I, Camarero S . Protein Engineering Approaches to Enhance Fungal Laccase Production in . Int J Mol Sci. 2021; 22(3). PMC: 7866195. DOI: 10.3390/ijms22031157. View

14.

Giardina P, Autore F, Faraco V, Festa G, Palmieri G, Piscitelli A . Structural characterization of heterodimeric laccases from Pleurotus ostreatus. Appl Microbiol Biotechnol. 2007; 75(6):1293-300. DOI: 10.1007/s00253-007-0954-4. View

15.

Brown N, Ries L, Goldman G . How nutritional status signalling coordinates metabolism and lignocellulolytic enzyme secretion. Fungal Genet Biol. 2014; 72:48-63. DOI: 10.1016/j.fgb.2014.06.012. View

16.

Penas M, Azparren G, Dominguez A, Sommer H, Ramirez L, Pisabarro A . Identification and functional characterisation of ctr1, a Pleurotus ostreatus gene coding for a copper transporter. Mol Genet Genomics. 2005; 274(4):402-9. DOI: 10.1007/s00438-005-0033-4. View

17.

Pezzella C, Lettera V, Piscitelli A, Giardina P, Sannia G . Transcriptional analysis of Pleurotus ostreatus laccase genes. Appl Microbiol Biotechnol. 2012; 97(2):705-17. DOI: 10.1007/s00253-012-3980-9. View

18.

Fernandez J, Wright J, Hartline D, Quispe C, Madayiputhiya N, Wilson R . Principles of carbon catabolite repression in the rice blast fungus: Tps1, Nmr1-3, and a MATE-family pump regulate glucose metabolism during infection. PLoS Genet. 2012; 8(5):e1002673. PMC: 3342947. DOI: 10.1371/journal.pgen.1002673. View

19.

Giardina P, Palmieri G, Scaloni A, Fontanella B, Faraco V, Cennamo G . Protein and gene structure of a blue laccase from Pleurotus ostreatus1. Biochem J. 1999; 341 ( Pt 3):655-63. PMC: 1220403. View

20.

Maestre-Reyna M, Liu W, Jeng W, Lee C, Hsu C, Wen T . Structural and functional roles of glycosylation in fungal laccase from Lentinus sp. PLoS One. 2015; 10(4):e0120601. PMC: 4388643. DOI: 10.1371/journal.pone.0120601. View