Leaf Extract Phytochemical Analysis, Cytotoxicity, In Vitro Antioxidant and Antidiabetic Activities

Overview

Journal Plants (Basel)

Date 2021 Dec 28

PMID 34961044

Citations 9

Authors

Wasim Ahmad

Mohd Amir

Adil Ahmad

Abuzer Ali

Amena Ali

Shadma Wahab

Harshita Abul Barkat

Mohammad Azam Ansari

Mohammad Sarafroz

Ayaz Ahmad

Md Abul Barkat

Prawez Alam

Affiliations

Soon will be listed here.

Abstract

For many years, () has been used medicinally and as a dietary supplement. Despite this, there are minimal research data on phytochemical properties and pharmacological effects. This study aimed to explore the phytoconstituents, cytotoxicity, glucose uptake, and antioxidant and antidiabetic potential of an alcoholic extract of leaf. The cytotoxicity of in HepG2 cells was tested in vitro, and the results revealed that it has strong cytocompatibility and cytoprotective properties. The extract's antioxidant activities were investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. Antioxidant potential was shown to be quite impressive. The enzymes α-amylase and α-glycosidase were found to be substantially inhibited by , with IC values of 46.21 and 42.07 mg/mL, respectively. In HepG2 cells, significantly reduced ROS levels that were elevated due to high glucose and enhanced glucose consumption ( < 0.05). These activities might be due to the enrichment of bioactive phytoconstituents analyzed chromatographically using GC/MS and HPLC. The findings of this study show that could be an effective restorative therapy for diabetes and related diseases.

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