Protective Effects of the Extracts of Barringtonia Racemosa Shoots Against Oxidative Damage in HepG2 Cells

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2016 Feb 4

PMID 26839752

Citations 10

Authors

Kin Weng Kong

Sarni Mat-Junit

Norhaniza Aminudin

Fouad Abdulrahman Hassan

Amin Ismail

Azlina Abdul Aziz

Affiliations

Soon will be listed here.

Abstract

Barringtonia racemosa is a tropical plant with medicinal values. In this study, the ability of the water extracts of the leaf (BLE) and stem (BSE) from the shoots to protect HepG2 cells against oxidative damage was studied. Five major polyphenolic compounds consisting of gallic acid, ellagic acid, protocatechuic acid, quercetin and kaempferol were identified using HPLC-DAD and ESI-MS. Cell viability assay revealed that BLE and BSE were non-cytotoxic (cell viabilities >80%) at concentration less than 250 µg/ml and 500 µg/ml, respectively. BLE and BSE improved cellular antioxidant status measured by FRAP assay and protected HepG2 cells against H2O2-induced cytotoxicity. The extracts also inhibited lipid peroxidation in HepG2 cells as well as the production of reactive oxygen species. BLE and BSE could also suppress the activities of superoxide dismutase and catalase during oxidative stress. The shoots of B. racemosa can be an alternative bioactive ingredient in the prevention of oxidative damage.

Citing Articles

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References

Hwang Y, Choi J, Choi J, Chung Y, Jeong H . Protective mechanisms of anthocyanins from purple sweet potato against tert-butyl hydroperoxide-induced hepatotoxicity. Food Chem Toxicol. 2011; 49(9):2081-9. DOI: 10.1016/j.fct.2011.05.021. View

Benzie I, Strain J . The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem. 1996; 239(1):70-6. DOI: 10.1006/abio.1996.0292. View

Kong K, Mat-Junit S, Ismail A, Aminudin N, Abdul-Aziz A . Polyphenols in Barringtonia racemosa and their protection against oxidation of LDL, serum and haemoglobin. Food Chem. 2013; 146:85-93. DOI: 10.1016/j.foodchem.2013.09.012. View

Mosmann T . Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65(1-2):55-63. DOI: 10.1016/0022-1759(83)90303-4. View

Choi J, Kim H, Choi Y, Ishihara A, Back K, Lee S . Cytoprotective activities of hydroxycinnamic acid amides of serotonin against oxidative stress-induced damage in HepG2 and HaCaT cells. Fitoterapia. 2010; 81(8):1134-41. DOI: 10.1016/j.fitote.2010.07.015. View

Alia M, Mateos R, Ramos S, Lecumberri E, Bravo L, Goya L . Influence of quercetin and rutin on growth and antioxidant defense system of a human hepatoma cell line (HepG2). Eur J Nutr. 2005; 45(1):19-28. DOI: 10.1007/s00394-005-0558-7. View

Yeum K, Russell R, Krinsky N, Aldini G . Biomarkers of antioxidant capacity in the hydrophilic and lipophilic compartments of human plasma. Arch Biochem Biophys. 2004; 430(1):97-103. DOI: 10.1016/j.abb.2004.03.006. View

Martin M, Ramos S, Mateos R, Izquierdo-Pulido M, Bravo L, Goya L . Protection of human HepG2 cells against oxidative stress by the flavonoid epicatechin. Phytother Res. 2009; 24(4):503-9. DOI: 10.1002/ptr.2961. View

Alia M, Ramos S, Mateos R, Granado-Serrano A, Bravo L, Goya L . Quercetin protects human hepatoma HepG2 against oxidative stress induced by tert-butyl hydroperoxide. Toxicol Appl Pharmacol. 2005; 212(2):110-8. DOI: 10.1016/j.taap.2005.07.014. View

10.

Martin M, Ramos S, Mateos R, Serrano A, Izquierdo-Pulido M, Bravo L . Protection of human HepG2 cells against oxidative stress by cocoa phenolic extract. J Agric Food Chem. 2008; 56(17):7765-72. DOI: 10.1021/jf801744r. View

11.

Shahrzad S, Aoyagi K, Winter A, Koyama A, Bitsch I . Pharmacokinetics of gallic acid and its relative bioavailability from tea in healthy humans. J Nutr. 2001; 131(4):1207-10. DOI: 10.1093/jn/131.4.1207. View

12.

Yoshihara D, Fujiwara N, Suzuki K . Antioxidants: benefits and risks for long-term health. Maturitas. 2010; 67(2):103-7. DOI: 10.1016/j.maturitas.2010.05.001. View

13.

Dai J, Mumper R . Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules. 2010; 15(10):7313-52. PMC: 6259146. DOI: 10.3390/molecules15107313. View

14.

Kobayashi H, Oikawa S, Hirakawa K, Kawanishi S . Metal-mediated oxidative damage to cellular and isolated DNA by gallic acid, a metabolite of antioxidant propyl gallate. Mutat Res. 2004; 558(1-2):111-20. DOI: 10.1016/j.mrgentox.2003.11.002. View

15.

Pieme C, Penlap V, Ngogang J, Costache M . In vitro cytotoxicity and antioxidant activities of five medicinal plants of Malvaceae family from Cameroon. Environ Toxicol Pharmacol. 2011; 29(3):223-8. DOI: 10.1016/j.etap.2010.01.003. View

16.

Hassan F, Ismail A, Abdulhamid A, Azlan A . Identification and quantification of phenolic compounds in bambangan (Mangifera pajang Kort.) peels and their free radical scavenging activity. J Agric Food Chem. 2011; 59(17):9102-11. DOI: 10.1021/jf201270n. View

17.

Boulton D, Walle U, Walle T . Extensive binding of the bioflavonoid quercetin to human plasma proteins. J Pharm Pharmacol. 1998; 50(2):243-9. DOI: 10.1111/j.2042-7158.1998.tb06183.x. View

18.

Buege J, Aust S . Microsomal lipid peroxidation. Methods Enzymol. 1978; 52:302-10. DOI: 10.1016/s0076-6879(78)52032-6. View

19.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

20.

Goya L, Mateos R, Bravo L . Effect of the olive oil phenol hydroxytyrosol on human hepatoma HepG2 cells. Protection against oxidative stress induced by tert-butylhydroperoxide. Eur J Nutr. 2007; 46(2):70-8. DOI: 10.1007/s00394-006-0633-8. View