Fractalkine-induced Microglial Vasoregulation Occurs Within the Retina and is Altered Early in Diabetic Retinopathy

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2021 Dec 14

PMID 34903661

Citations 40

Authors

Samuel A Mills

Andrew I Jobling

Michael A Dixon

Bang V Bui

Kirstan A Vessey

Joanna A Phipps

Ursula Greferath

Gene Venables

Vickie H Y Wong

Connie H Y Wong

Zheng He

Flora Hui

James C Young

Josh Tonc

Elena Ivanova

Botir T Sagdullaev

Erica L Fletcher

Affiliations

Soon will be listed here.

Abstract

Local blood flow control within the central nervous system (CNS) is critical to proper function and is dependent on coordination between neurons, glia, and blood vessels. Macroglia, such as astrocytes and Müller cells, contribute to this neurovascular unit within the brain and retina, respectively. This study explored the role of microglia, the innate immune cell of the CNS, in retinal vasoregulation, and highlights changes during early diabetes. Structurally, microglia were found to contact retinal capillaries and neuronal synapses. In the brain and retinal explants, the addition of fractalkine, the sole ligand for monocyte receptor Cx3cr1, resulted in capillary constriction at regions of microglial contact. This vascular regulation was dependent on microglial Cx3cr1 involvement, since genetic and pharmacological inhibition of Cx3cr1 abolished fractalkine-induced constriction. Analysis of the microglial transcriptome identified several vasoactive genes, including angiotensinogen, a constituent of the renin-angiotensin system (RAS). Subsequent functional analysis showed that RAS blockade via candesartan abolished microglial-induced capillary constriction. Microglial regulation was explored in a rat streptozotocin (STZ) model of diabetic retinopathy. Retinal blood flow was reduced after 4 wk due to reduced capillary diameter and this was coincident with increased microglial association. Functional assessment showed loss of microglial-capillary response in STZ-treated animals and transcriptome analysis showed evidence of RAS pathway dysregulation in microglia. While candesartan treatment reversed capillary constriction in STZ-treated animals, blood flow remained decreased likely due to dilation of larger vessels. This work shows microglia actively participate in the neurovascular unit, with aberrant microglial-vascular function possibly contributing to the early vascular compromise during diabetic retinopathy.

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