Osteogenic Property Regulation of Stem Cells by a Hydroxyapatite 3D-Hybrid Scaffold With Cancellous Bone Structure

Overview

Journal Front Chem

Specialty Chemistry

Date 2021 Dec 6

PMID 34869241

Citations 7

Authors

He Xia

Lun Dong

Min Hao

Yuan Wei

Jiazhi Duan

Xin Chen

Liyang Yu

Haijun Li

Yuanhua Sang

Hong Liu

Affiliations

Soon will be listed here.

Abstract

Cancellous bone plays an indispensable role in the skeletal system due to its various functions and high porosity. In this work, chitosan and hydroxyapatite nanowires (CS@HAP NWs) hybrid nanostructured scaffolds with suitable mechanical properties, high porosity and a fine porous structure were prepared to simulate the 3-dimensional structure of cancellous bone. The 3D-hybrid scaffolds promote cell adhesion and the migration of human adipose-derived stem cells (hADSCs) inside the scaffolds. The cavities in the scaffolds provide space for the hADSCs proliferation and differentiation. Moreover, the various contents of HAP and the induced mechanical property changes regulate the differentiation of hADSCs toward osteoblasts. Overall, cellular fate regulation of hADSCs via rationally engineered HAP-based hybrid scaffolds is a facile and effective approach for bone tissue engineering.

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References

Annamalai R, Hong X, Schott N, Tiruchinapally G, Levi B, Stegemann J . Injectable osteogenic microtissues containing mesenchymal stromal cells conformally fill and repair critical-size defects. Biomaterials. 2019; 208:32-44. PMC: 6500486. DOI: 10.1016/j.biomaterials.2019.04.001. View

Wang X, Nie Z, Chang J, Lu M, Kang Y . Multiple channels with interconnected pores in a bioceramic scaffold promote bone tissue formation. Sci Rep. 2021; 11(1):20447. PMC: 8516977. DOI: 10.1038/s41598-021-00024-z. View

Oftadeh R, Perez-Viloria M, Villa-Camacho J, Vaziri A, Nazarian A . Biomechanics and mechanobiology of trabecular bone: a review. J Biomech Eng. 2014; 137(1). PMC: 5101038. DOI: 10.1115/1.4029176. View

Levengood S, Zhang M . Chitosan-based scaffolds for bone tissue engineering. J Mater Chem B. 2014; 2(21):3161-3184. PMC: 4078888. DOI: 10.1039/C4TB00027G. View

Wang S, Jiang D, Zhang Z, Chen Y, Yang Z, Zhang J . Biomimetic Nanosilica-Collagen Scaffolds for In Situ Bone Regeneration: Toward a Cell-Free, One-Step Surgery. Adv Mater. 2019; 31(49):e1904341. DOI: 10.1002/adma.201904341. View

Lee J, Lee S, Ahmad T, Perikamana S, Lee J, Kim E . Human adipose-derived stem cell spheroids incorporating platelet-derived growth factor (PDGF) and bio-minerals for vascularized bone tissue engineering. Biomaterials. 2020; 255:120192. DOI: 10.1016/j.biomaterials.2020.120192. View

Brennan M, Monahan D, Brulin B, Gallinetti S, Humbert P, Tringides C . Biomimetic versus sintered macroporous calcium phosphate scaffolds enhanced bone regeneration and human mesenchymal stromal cell engraftment in calvarial defects. Acta Biomater. 2021; 135:689-704. DOI: 10.1016/j.actbio.2021.09.007. View

Lin H, Sohn J, Shen H, Langhans M, Tuan R . Bone marrow mesenchymal stem cells: Aging and tissue engineering applications to enhance bone healing. Biomaterials. 2018; 203:96-110. PMC: 6733253. DOI: 10.1016/j.biomaterials.2018.06.026. View

Cui Z, Kim S, Baljon J, Wu B, Aghaloo T, Lee M . Microporous methacrylated glycol chitosan-montmorillonite nanocomposite hydrogel for bone tissue engineering. Nat Commun. 2019; 10(1):3523. PMC: 6684526. DOI: 10.1038/s41467-019-11511-3. View

10.

Gaihre B, Jayasuriya A . Comparative investigation of porous nano-hydroxyapaptite/chitosan, nano-zirconia/chitosan and novel nano-calcium zirconate/chitosan composite scaffolds for their potential applications in bone regeneration. Mater Sci Eng C Mater Biol Appl. 2018; 91:330-339. PMC: 6061966. DOI: 10.1016/j.msec.2018.05.060. View

11.

Zhang Y, Zhu Y, Xiong Z, Wu J, Chen F . Bioinspired Ultralight Inorganic Aerogel for Highly Efficient Air Filtration and Oil-Water Separation. ACS Appl Mater Interfaces. 2018; 10(15):13019-13027. DOI: 10.1021/acsami.8b02081. View

12.

Jo Y, Choi B, Kim C, Cha H . Diatom-Inspired Silica Nanostructure Coatings with Controllable Microroughness Using an Engineered Mussel Protein Glue to Accelerate Bone Growth on Titanium-Based Implants. Adv Mater. 2017; 29(46). DOI: 10.1002/adma.201704906. View

13.

Komori T . Runx2, an inducer of osteoblast and chondrocyte differentiation. Histochem Cell Biol. 2018; 149(4):313-323. DOI: 10.1007/s00418-018-1640-6. View

14.

Hao M, Zhang Z, Liu C, Tian Y, Duan J, He J . Hydroxyapatite Nanorods Function as Safe and Effective Growth Factors Regulating Neural Differentiation and Neuron Development. Adv Mater. 2021; 33(33):e2100895. DOI: 10.1002/adma.202100895. View

15.

Zhong W, Li J, Hu C, Quan Z, Jiang D . Enhancement of the bone-implant interface by applying a plasma-sprayed titanium coating on nanohydroxyapatite/polyamide66 implants in a rabbit model. Sci Rep. 2021; 11(1):19971. PMC: 8497622. DOI: 10.1038/s41598-021-99494-4. View

16.

Kim J, El-Fiqi A, Kim H . Synergetic Cues of Bioactive Nanoparticles and Nanofibrous Structure in Bone Scaffolds to Stimulate Osteogenesis and Angiogenesis. ACS Appl Mater Interfaces. 2016; 9(3):2059-2073. DOI: 10.1021/acsami.6b12089. View

17.

Wittig N, Palle J, Ostergaard M, Frolich S, Birkbak M, Spiers K . Bone Biomineral Properties Vary across Human Osteonal Bone. ACS Nano. 2019; 13(11):12949-12956. DOI: 10.1021/acsnano.9b05535. View

18.

Jiang S, Lyu C, Zhao P, Li W, Kong W, Huang C . Cryoprotectant enables structural control of porous scaffolds for exploration of cellular mechano-responsiveness in 3D. Nat Commun. 2019; 10(1):3491. PMC: 6677882. DOI: 10.1038/s41467-019-11397-1. View

19.

Ma B, Zhang S, Liu F, Duan J, Wang S, Han J . One-Dimensional Hydroxyapatite Nanostructures with Tunable Length for Efficient Stem Cell Differentiation Regulation. ACS Appl Mater Interfaces. 2017; 9(39):33717-33727. DOI: 10.1021/acsami.7b13313. View

20.

Liu C, Zhai H, Zhang Z, Li Y, Xu X, Tang R . Cells Recognize and Prefer Bone-like Hydroxyapatite: Biochemical Understanding of Ultrathin Mineral Platelets in Bone. ACS Appl Mater Interfaces. 2016; 8(44):29997-30004. DOI: 10.1021/acsami.6b10374. View